表皮生长因子和胰岛素会使正常鸡心脏间充质细胞像被劳氏肉瘤病毒感染的对应细胞一样增殖。
Epidermal growth factor and insulin cause normal chicken heart mesenchymal cells to proliferate like their Rous sarcoma virus-infected counterparts.
作者信息
Balk S D, Shiu R P, LaFleur M M, Young L L
出版信息
Proc Natl Acad Sci U S A. 1982 Feb;79(4):1154-7. doi: 10.1073/pnas.79.4.1154.
Abstract
Normal chicken heart mesenchymal cells at low culture density are proliferatively quiescent in a physiological culture medium containing heparinized, heat-inactivated, chicken plasma at 10%. Rous sarcoma virus-infected chicken heart mesenchymal cells, on the other hand, proliferate maximally in this same medium, undergoing a 60-fold increase in cell number during 4 days of exponential growth. When normal heart mesenchymal cells are cultured for 4 days in the presence of epidermal growth factor at 1 micrograms/ml they undergo a 16-fold increase in number, with graded responses to lower concentrations of the factor. In the presence of insulin at 10 micrograms/ml, normal heart mesenchymal cells multiply by a factor of 3 over a 4-day period. The addition of epidermal growth factor (1 microgram/ml) and insulin (10 micrograms/ml) to cultures of normal chicken heart mesenchymal cells causes these cells to proliferate at a rate comparable to that of their RSV-infected counterparts.
摘要
在含有10%肝素化、热灭活鸡血浆的生理培养基中，低密度培养的正常鸡心脏间充质细胞处于增殖静止状态。另一方面，感染劳氏肉瘤病毒的鸡心脏间充质细胞在相同培养基中能最大程度地增殖，在指数生长期的4天内细胞数量增加60倍。当正常心脏间充质细胞在1微克/毫升表皮生长因子存在的情况下培养4天时，其数量增加16倍，对较低浓度的该因子有分级反应。在10微克/毫升胰岛素存在的情况下，正常心脏间充质细胞在4天内数量增加3倍。向正常鸡心脏间充质细胞培养物中添加表皮生长因子（1微克/毫升）和胰岛素（10微克/毫升）会使这些细胞以与其感染劳氏肉瘤病毒的对应细胞相当的速率增殖。
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Proc Natl Acad Sci U S A. 1982 Feb;79(4):1154-7. doi: 10.1073/pnas.79.4.1154.
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引用本文的文献
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Phorbol 12-myristate 13-acetate, ionomycin or ouabain, and raised extracellular magnesium induce proliferation of chicken heart mesenchymal cells.佛波醇12 -肉豆蔻酸酯13 -乙酸酯、离子霉素或哇巴因，以及提高细胞外镁离子浓度可诱导鸡心脏间充质细胞增殖。
Proc Natl Acad Sci U S A. 1984 Oct;81(20):6418-21. doi: 10.1073/pnas.81.20.6418.
2
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