Warner A E, Guthrie S C, Gilula N B
Nature. 1984;311(5982):127-31. doi: 10.1038/311127a0.
Antibodies to the major protein of rat liver gap junctions, molecular weight 27,000 (27K), have been microinjected into one identified cell of 8-cell stage Xenopus embryos. This treatment selectively disrupts both dye transfer and electrical coupling between the progeny cells. These results provide evidence that the 27K protein is an integral component of the cell-to-cell junctional channel. The disruption of junctional communication at early stages results in specific developmental defects, suggesting that blocking intercellular communication can have a pronounced influence on embryonic development.
已将针对大鼠肝脏缝隙连接主要蛋白（分子量27,000，即27K）的抗体显微注射到8细胞期非洲爪蟾胚胎的一个已识别细胞中。这种处理选择性地破坏了子代细胞之间的染料转移和电偶联。这些结果证明27K蛋白是细胞间连接通道的一个组成成分。早期阶段连接通讯的破坏会导致特定的发育缺陷，这表明阻断细胞间通讯会对胚胎发育产生显著影响。
