Ueda N, Suzuki Y, Utsumi M, Obara T, Okamura K, Namiki M
Peptides. 1984 Mar-Apr;5(2):423-8. doi: 10.1016/0196-9781(84)90247-x.
A pancreatic ductal carcinoma was established as a transplantable tumor line in an inbred strain of Syrian golden hamsters. Intracellular recordings of membrane potentials and input resistance were made from cultured cells obtained from the transplanted tumors using indwelling glass microelectrode. The mean value of the resting membrane potential was -46.5 +/- 1.8 mV (S.E.) (n = 13), while the mean resting input resistance was 21.2 +/- 4.3 M omega (S.E.) (N = 13). Dibutyryl cyclic AMP (2 X 10(-3)M) caused a marked hyperpolarization of about 30 mV accompanied by a reduction of input resistance. The transplantable tumor and its cultured cell line developed in this study have demonstrated their effectiveness as a reliable experimental model for use in pancreatic cancer research.
在近交系叙利亚金仓鼠中建立了一种可移植的胰腺导管癌肿瘤系。使用植入式玻璃微电极，对从移植肿瘤中获取的培养细胞进行膜电位和输入电阻的细胞内记录。静息膜电位的平均值为-46.5±1.8 mV（标准误）（n = 13），而静息输入电阻的平均值为21.2±4.3 MΩ（标准误）（N = 13）。二丁酰环磷腺苷（2×10⁻³M）引起约30 mV的明显超极化，同时输入电阻降低。本研究中建立的可移植肿瘤及其培养细胞系已证明其作为胰腺癌研究可靠实验模型的有效性。
