Henry I, Uzan G, Weil D, Nicolas H, Kaplan J C, Marguerie C, Kahn A, Junien C
Am J Hum Genet. 1984 Jul;36(4):760-8.
We used cloned cDNA probes for the A alpha-, B beta-, and gamma-chains of human fibrinogen and Southern blotting techniques to analyze DNA from a series of rodent X human somatic cell hybrids for the presence of specific fibrinogen-related sequences. Our results provide evidence for the assignment of the three genes for fibrinogen to chromosome 4. Moreover, by direct gene-dosage determination in two patients with chromosome 4 unbalanced rearrangements, we refined the regional chromosomal assignment to 4q2, thus suggesting that these three genes whose expression is coordinately regulated are closely linked.
我们使用人纤维蛋白原Aα、Bβ和γ链的克隆cDNA探针以及Southern印迹技术，分析了一系列啮齿动物×人类体细胞杂种的DNA，以检测特定纤维蛋白原相关序列的存在。我们的结果为将纤维蛋白原的三个基因定位于4号染色体提供了证据。此外，通过对两名患有4号染色体不平衡重排患者的直接基因剂量测定，我们将区域染色体定位细化到4q2，从而表明这三个表达受到协调调控的基因紧密连锁。
