A kinetic isotope dilution assay for glycerol.
作者信息
Bell J D, Buxton I L, Brunton L L
出版信息
Anal Biochem. 1984 Jun;139(2):305-8. doi: 10.1016/0003-2697(84)90008-3.
PMID:6089605
Abstract
Using glycerol kinase and [3H]glycerol, a kinetic isotope dilution assay for glycerol has been developed. Reactant and product are separated by stepwise elution from QAE-Sephadex. This assay is sensitive to as little as 100 pmol of glycerol, avoids numerous drawbacks of the traditional fluorescent assay, and readily detects glycerol production by fewer than 10(5) cardiomyocytes.
摘要
Zotero 插件