Quinnan G V, Delery M, Rook A H, Frederick W R, Epstein J S, Manischewitz J F, Jackson L, Ramsey K M, Mittal K, Plotkin S A
Ann Intern Med. 1984 Oct;101(4):478-83. doi: 10.7326/0003-4819-101-4-478.
The Towne strain cytomegalovirus and a low-passage strain, Toledo-1, were compared for virulence and immunogenicity in healthy adult male subjects to determine the suitability of the Towne strain for vaccination. Five seropositive subjects who received the Toledo-1 strain developed infections ranging in severity from laboratory abnormalities to mild mononucleosis syndromes (mean incubation, 4.7 weeks). None of the four seronegative subjects receiving the Towne strain developed systemic infection, but all developed delayed local reactions at the injection sites. All subjects developed cytotoxic lymphocyte responses specific to cytomegalovirus, usually HLA-restricted, but these were of greater magnitude and duration in the Toledo-1 recipients. The latter also developed natural killer cell and interferon responses, atypical lymphocytosis, inversion of helper/suppressor cell ratios, and depressed responses to T-cell mitogens, none of which occurred in Towne strain recipients. The results further substantiate the avirulence and immunogenicity of the Towne strain cytomegalovirus.
在健康成年男性受试者中，比较了汤氏株巨细胞病毒和低传代株托莱多-1的毒力和免疫原性，以确定汤氏株用于疫苗接种的适用性。五名接受托莱多-1株的血清阳性受试者发生了从实验室异常到轻度单核细胞增多症综合征不等的感染（平均潜伏期4.7周）。四名接受汤氏株的血清阴性受试者均未发生全身感染，但所有受试者在注射部位均出现了延迟性局部反应。所有受试者均产生了针对巨细胞病毒的细胞毒性淋巴细胞反应，通常受HLA限制，但在接受托莱多-1株的受试者中，这些反应的强度和持续时间更大。后者还产生了自然杀伤细胞和干扰素反应、非典型淋巴细胞增多、辅助/抑制细胞比例倒置以及对T细胞有丝分裂原的反应降低，而这些在接受汤氏株的受试者中均未发生。结果进一步证实了汤氏株巨细胞病毒的无毒力和免疫原性。
