Plotkin S A, Starr S E, Friedman H M, Gönczöl E, Weibel R E
Children's Hospital of Philadelphia, PA 19104.
J Infect Dis. 1989 May;159(5):860-5. doi: 10.1093/infdis/159.5.860.
To test the protective effect of Towne live attenuated human cytomegalovirus (HCMV) vaccine in normal individuals, we developed a parenteral challenge consisting of a low-passage isolate (Toledo stain) inoculated subcutaneously in graded doses. This challenge virus caused a mild mononucleosis syndrome in seronegative individuals at doses of 10 or 100 pfu. The illness was accompanied by atypical lymphocytosis, raised hepatic enzymes, excretion of HCMV and HCMV-specific immune responses. Naturally seropositive volunteers also developed clinical and laboratory evidence of infection after challenge with 1,000 pfu of Toledo but resisted 10 or 100 pfu. Volunteers who had been vaccinated 1 y earlier also were resistant to disease caused by 10 or 100 pfu of Toledo, although some were asymptomatically infected by the 100 pfu dose. Vaccine-induced immunity to HCMV was as complete as naturally induced immunity when the challenge dose of Toledo was 10 pfu.
为了测试Towne减毒活人类巨细胞病毒（HCMV）疫苗在正常个体中的保护作用，我们开发了一种肠胃外激发试验，该试验由皮下接种不同剂量的低传代分离株（托莱多毒株）组成。这种激发病毒在血清阴性个体中，剂量为10或100个噬斑形成单位（pfu）时，会引发轻度单核细胞增多症综合征。该病症伴有非典型淋巴细胞增多、肝酶升高、HCMV排泄以及HCMV特异性免疫反应。自然血清阳性的志愿者在用1000 pfu托莱多毒株激发后，也出现了感染的临床和实验室证据，但对10或100 pfu有抵抗力。1年前接种过疫苗的志愿者对10或100 pfu托莱多毒株引起的疾病也有抵抗力，尽管有些人被100 pfu剂量无症状感染。当托莱多毒株的激发剂量为10 pfu时，疫苗诱导的对HCMV的免疫力与自然诱导的免疫力一样完全。
