Ohno T, Yoshii K, Kurihara K
Brain Res. 1984 Sep 17;310(1):13-21. doi: 10.1016/0006-8993(84)90005-2.
A quantitative method of cross-adaptation was developed to explore the difference in the receptor sites for various amino acids in the carp olfactory receptors. The olfactory responses were measured by recording the stimulant-induced waves from the olfactory bulb. Cross-adaptation was carried out by varying concentrations of amino acids in a wide range. Typical examples of the results obtained are as follows. The response to Thr after Ser was decreased with increasing concentration of Ser applied first and reached the spontaneous level, while that to Thr after Glu was decreased to 77% of the level of the original response with increasing Glu concentration and stayed this constant level with a further increase in Glu concentration. Application of the amino acids in the reverse order gave essentially similar results. Such types of experiments were carried out between various pairs of amino acids. The results obtained suggested that amino acids of very close species (e.g. Thr and Ser, Asp and Glu, Tyr and Phe) stimulate the same respective receptor sites and that amino acids of most other pairs stimulate more or less different receptor sites, although there exist the receptor sites stimulated commonly by both amino acids of one pair. It was concluded that the carp olfactory cells have many different receptor sites stimulated only by one species of amino acid and its close analogues.
开发了一种交叉适应的定量方法，以探究鲤鱼嗅觉感受器中各种氨基酸受体位点的差异。通过记录嗅球中刺激诱发的波来测量嗅觉反应。交叉适应是通过在很宽的范围内改变氨基酸浓度来进行的。获得的典型结果如下。先用丝氨酸处理后，苏氨酸的反应随着丝氨酸浓度的增加而降低，并达到自发水平，而先用谷氨酸处理后，苏氨酸的反应随着谷氨酸浓度的增加而降低至原始反应水平的77%，并在谷氨酸浓度进一步增加时保持这一恒定水平。以相反顺序施用氨基酸得到基本相似的结果。在各种氨基酸对之间进行了此类实验。获得的结果表明，亲缘关系非常近的氨基酸（如苏氨酸和丝氨酸、天冬氨酸和谷氨酸、酪氨酸和苯丙氨酸）刺激相同的各自受体位点，而大多数其他对的氨基酸刺激或多或少不同的受体位点，尽管存在一对氨基酸中的两种氨基酸共同刺激的受体位点。得出的结论是，鲤鱼嗅觉细胞有许多不同的受体位点，仅由一种氨基酸及其密切类似物刺激。
