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针对合成寡肽的抗体能够鉴定出由酵母cob-box基因第二个内含子编码的mRNA成熟酶。

Antibodies against synthetic oligopeptides allow identification of the mRNA-maturase encoded by the second intron of the yeast cob-box gene.

作者信息

Guiso N, Dreyfus M, Siffert O, Danchin A, Spyridakis A, Gargouri A, Claisse M, Slonimski P P

出版信息

EMBO J. 1984 Aug;3(8):1769-72. doi: 10.1002/j.1460-2075.1984.tb02044.x.

DOI:10.1002/j.1460-2075.1984.tb02044.x
PMID:6090125
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC557594/
Abstract

Genetic and biochemical evidence has strongly suggested that several introns located in yeast mitochondrial genes specifying apocytochrome b or cytochrome oxidase encode trans-acting proteins (termed mRNA-maturases) responsible for splicing the cognate intron and maturation of the mRNA. We have chemically synthesized three oligopeptides, predicted from the DNA sequence of the open reading frame (ORF) present in the second intron of the cob-box gene, and raised antibodies against them. These antibodies have allowed us to identify a protein of 42 kd as the product translated from the ORF of the wild-type intron. In two splicing-deficient mutants this protein is replaced by shorter polypeptides whose lengths and antigenic properties are in full agreement with the positions of TAA codons established by the DNA sequence of the intron's ORF.

摘要

遗传学和生物化学证据有力地表明，酵母线粒体基因中几个特定于脱辅基细胞色素b或细胞色素氧化酶的内含子编码反式作用蛋白（称为mRNA成熟酶），这些蛋白负责同源内含子的剪接和mRNA的成熟。我们根据cob-box基因第二个内含子中存在的开放阅读框（ORF）的DNA序列预测，化学合成了三种寡肽，并制备了针对它们的抗体。这些抗体使我们能够鉴定出一种42kd的蛋白质，它是从野生型内含子的ORF翻译而来的产物。在两个剪接缺陷型突变体中，这种蛋白质被较短的多肽所取代，这些多肽的长度和抗原特性与内含子ORF的DNA序列确定的TAA密码子位置完全一致。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1c3/557594/1bcd9131350d/emboj00312-0105-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1c3/557594/1bcd9131350d/emboj00312-0105-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1c3/557594/1bcd9131350d/emboj00312-0105-a.jpg

相似文献

1
Antibodies against synthetic oligopeptides allow identification of the mRNA-maturase encoded by the second intron of the yeast cob-box gene.针对合成寡肽的抗体能够鉴定出由酵母cob-box基因第二个内含子编码的mRNA成熟酶。
EMBO J. 1984 Aug;3(8):1769-72. doi: 10.1002/j.1460-2075.1984.tb02044.x.
2
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引用本文的文献

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本文引用的文献

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Expression of the split gene cob in yeast: evidence for a precursor of a "maturase" protein translated from intron 4 and preceding exons.裂合基因cob在酵母中的表达：从内含子4及之前的外显子翻译出的“成熟酶”蛋白前体的证据。
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Long range control circuits within mitochondria and between nucleus and mitochondria. II. Genetic and biochemical analyses of suppressors which selectively alleviate the mitochondrial intron mutations.线粒体内部以及细胞核与线粒体之间的远程控制电路。II. 对选择性缓解线粒体内含子突变的抑制子的遗传和生化分析。
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Mol Cell Biol. 1995 Feb;15(2):780-9. doi: 10.1128/MCB.15.2.780.
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The chloroplast ribosomal intron of Chlamydomonas reinhardii codes for a polypeptide related to mitochondrial maturases.莱茵衣藻的叶绿体核糖体内含子编码一种与线粒体成熟酶相关的多肽。
Nucleic Acids Res. 1985 Feb 11;13(3):975-84. doi: 10.1093/nar/13.3.975.
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Efficient splicing of two yeast mitochondrial introns controlled by a nuclear-encoded maturase.
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Expression of the mitochondrial split gene coding for cytochrome oxidase subunit I in S. cerevisiae: RNA splicing pathway.酿酒酵母中编码细胞色素氧化酶亚基I的线粒体分裂基因的表达：RNA剪接途径。
Curr Genet. 1986;11(1):55-63. doi: 10.1007/BF00389426.
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Analysis of HPV-1 E4 gene expression using epitope-defined antibodies.使用表位特异性抗体分析人乳头瘤病毒1型E4基因表达
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Splice points of the third intron in the yeast mitochondrial cytochrome b gene.
酵母线粒体细胞色素b基因中第三个内含子的剪接位点。
Cell. 1981 Nov;27(1 Pt 2):12-4. doi: 10.1016/0092-8674(81)90355-x.
4
Expression of the "split gene" COB in yeast mtDNA. Translation of intervening sequences in mutant strains.酵母线粒体DNA中“断裂基因”COB的表达。突变菌株中介入序列的翻译。
J Biol Chem. 1981 Apr 10;256(7):3525-31.
5
Sequence of introns and flanking exons in wild-type and box3 mutants of cytochrome b reveals an interlaced splicing protein coded by an intron.细胞色素b野生型和box3突变体中内含子及侧翼外显子的序列揭示了一个由内含子编码的交错剪接蛋白。
Cell. 1980 Nov;22(2 Pt 2):333-48. doi: 10.1016/0092-8674(80)90344-x.
6
A pathway of cytochrome b mRNA processing in yeast mitochondria: specific splicing steps and an intron-derived circular DNA.酵母线粒体中细胞色素b信使核糖核酸加工的一条途径：特定的剪接步骤和一种内含子衍生的环状脱氧核糖核酸。
Cell. 1980 Feb;19(2):321-9. doi: 10.1016/0092-8674(80)90506-1.
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Functional domains in introns: trans-acting and cis-acting regions of intron 4 of the cob gene.内含子中的功能结构域：cob基因第4内含子的反式作用和顺式作用区域
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Location and structure of the var1 gene on yeast mitochondrial DNA: nucleotide sequence of the 40.0 allele.酵母线粒体DNA上var1基因的定位与结构：40.0等位基因的核苷酸序列
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9
Antibodies against a fused 'lacZ-yeast mitochondrial intron' gene product allow identification of the mRNA maturase encoded by the fourth intron of the yeast cob-box gene.针对融合的“lacZ-酵母线粒体内含子”基因产物的抗体，能够鉴定出由酵母cob-box基因的第四个内含子所编码的mRNA成熟酶。
EMBO J. 1984 Jul;3(7):1567-72. doi: 10.1002/j.1460-2075.1984.tb02012.x.
10
Antibodies against synthetic peptides reveal that the unidentified reading frame A6L, overlapping the ATPase 6 gene, is expressed in human mitochondria.针对合成肽的抗体表明，与ATPase 6基因重叠的未鉴定读码框A6L在人线粒体中表达。
Cell. 1983 Apr;32(4):1269-77. doi: 10.1016/0092-8674(83)90308-2.