Matsuoka M, Matsubara M, Kakehi M, Imanaka T
Department of Biotechnology, Faculty of Engineering, Osaka University, Japan.
Curr Genet. 1994 Nov-Dec;26(5-6):377-81. doi: 10.1007/BF00309922.
A mitochondrial cox3 gene in the alkane yeast, Yarrowia lipolytica, encodes a subunit-3 protein of cytochrome c oxidase, and contains a 1044 base-pair-long intron, as compared with the corresponding intronless gene in Saccharomyces cerevisiae. The intron belongs to a group I intron as determined by the cDNA sequence for the splicing sites as well as the predicted RNA secondary structure. Remarkably, this intron could code for a protein of 206 amino-acid residues which showed 63% similarity with an RNA maturase encoded by the second intron of the mitochondrial apocytochrome b gene in S. cerevisiae. Both introns occurred within the conserved exon sequence, 5'-TT(G/C)AGGTGC-3', suggesting the possible transposition of a common ancestral intron.
解脂耶氏酵母（Yarrowia lipolytica）这种烷烃酵母中的线粒体细胞色素c氧化酶亚基3（cox3）基因编码细胞色素c氧化酶的一个亚基3蛋白，与酿酒酵母（Saccharomyces cerevisiae）中相应的无内含子基因相比，它含有一个长度为1044个碱基对的内含子。根据剪接位点的cDNA序列以及预测的RNA二级结构确定，该内含子属于I类内含子。值得注意的是，这个内含子能够编码一个由206个氨基酸残基组成的蛋白质，该蛋白质与酿酒酵母线粒体脱辅基细胞色素b基因第二个内含子编码的RNA成熟酶具有63%的相似性。两个内含子都出现在保守的外显子序列5'-TT(G/C)AGGTGC-3'内，这表明可能存在一个共同祖先内含子的转座。
