Anziano P Q, Hanson D K, Mahler H R, Perlman P S
Cell. 1982 Oct;30(3):925-32. doi: 10.1016/0092-8674(82)90297-5.
We have sequenced the mutational changes in eight mutants in the open reading frame of intron 4 of the cob gene on yeast mitochondrial DNA. Three have a cis-acting splicing defect, while the other inactivate a trans-recessive intron domain that specifies a trans-acting splicing factor. From phenotypic evidence, including analyses of the allele-specific extra proteins, we have identified a protein (P27) encoded wholly within the intron that appears to be the intron 4 splicing factor (maturase). The evidence suggests that P27 is a secondary translation product resulting from the proteolytic cleavage of a larger precursor encoded by exon and intron sequences, but an alternative model, in which P27 is a primary translation product, has not been ruled out.
我们已对酵母线粒体DNA上cob基因第4内含子开放阅读框中的八个突变体的突变变化进行了测序。其中三个具有顺式作用剪接缺陷，而另外几个则使一个反式隐性内含子结构域失活，该结构域可指定一个反式作用剪接因子。从包括等位基因特异性额外蛋白质分析在内的表型证据中，我们鉴定出一种完全由内含子编码的蛋白质（P27），它似乎是第4内含子剪接因子（成熟酶）。证据表明P27是由外显子和内含子序列编码的较大前体蛋白经蛋白水解切割产生的二级翻译产物，但P27是一级翻译产物的另一种模型也未被排除。
