二乙基二硫代氨基甲酸盐（DDC）与适度高温对受光子照射后的中国仓鼠（V79）细胞存活及DNA链断裂修复的联合作用

Combined effect of diethyldithiocarbamate (DDC) and modest hyperthermia on Chinese hamster (V79) cell survival and DNA strand break repair following photon irradiation.

作者信息

Wagner H, Parkinson D R, Madoc-Jones H, Sternick E S, Vrusho K, Krasin F

出版信息

Int J Radiat Oncol Biol Phys. 1984 Sep;10(9):1575-9. doi: 10.1016/0360-3016(84)90506-6.

Abstract

Previous studies from our institution have shown that 10(-4) M DDC enhances the effects of radiation and of hyperthermia treatment at 43 degrees C on the killing of Chinese hamster (DON) cells. We herein report studies on the combined effect seen at more modest temperatures (41 degrees C) which can be achieved in humans by whole body heating without the need for general anesthesia. Treatment of V79 cells with DDC for 60 minutes at 37 degrees C or 41 degrees C had minimal toxicity at concentrations up to 5 X 10(-5) M. When cells were irradiated with single doses up to 1000 rad (137Cs, 350 rad/min), pre-incubation with 10(-4) M DDC had no effect on cell survival at 37 degrees C, but markedly decreased survival at 41 degrees C (D37 = 475 rad without DDC, 270 rad with DDC). The mechanism of this increased cell killing is not known. We observed, however, that there is no repair of DNA single strand breaks in DNA from irradiated V79 cells previously held at 41 degrees C in the presence of 10(-4) M DDC. Without DDC, repair of SSB was similar at 37 degrees C and 41 degrees C.

摘要

我们机构之前的研究表明，10⁻⁴ M的二硫代苏糖醇（DDC）可增强辐射以及43℃热疗对中国仓鼠（DON）细胞的杀伤作用。我们在此报告关于在更适度温度（41℃）下联合作用的研究，这种温度可通过全身加热在人体实现，无需全身麻醉。在37℃或41℃用DDC处理V79细胞60分钟，浓度高达5×10⁻⁵ M时毒性极小。当细胞接受高达1000拉德（¹³⁷Cs，350拉德/分钟）的单次照射时，用10⁻⁴ M DDC预孵育在37℃时对细胞存活无影响，但在41℃时显著降低了存活率（无DDC时D37 = 475拉德，有DDC时为270拉德）。这种细胞杀伤增加的机制尚不清楚。然而，我们观察到，在10⁻⁴ M DDC存在下，先前在41℃保存的受照射V79细胞的DNA中，DNA单链断裂没有修复。没有DDC时，37℃和41℃时单链断裂的修复情况相似。