Allalunis-Turner M J, Chapman J D
Int J Radiat Oncol Biol Phys. 1984 Sep;10(9):1569-73. doi: 10.1016/0360-3016(84)90505-4.
The radioprotective action of DDC on normal hematopoietic tissue in mice was evaluated. An increase in CFU-S and CFU-GM in DDC treated unirradiated control mice was consistently observed. When post-irradiation survival of CFU-S and CFU-GM from DDC treated animals was normalized to account for this observed increase, protection factors ranging from 0.9 to 1.6 were observed. These protection factors are significantly lower than those reported by others. Maximum radioprotection was observed when irradiations were performed 15 to 30 min after DDC treatment; maximum increase in GFU-GM occurred within one to two hr following treatment. The increase in CFU-GM is analogous to that observed in animals treated with endotoxin, a non-thiol radioprotector. When C3H/HeJ mice, which are genetically incapable of responding to endotoxin, were challenged with DDC, an average CFU-GM increase of 1.7 times was observed, suggesting that the stimulatory effects of DDC were not due to endotoxin contamination. DDC was administered daily for three days before irradiation and little or no increase in CFU-GM and no radioprotection was observed, suggesting that the marrow can become refractory to DDC. When WR-2721 was tested in similar studies, a dose-modifying radioprotection was observed, with no significant non-specific stimulation of hematopoietic cells.
评估了二硫代二乙酰胺（DDC）对小鼠正常造血组织的辐射防护作用。在未受照射的DDC处理对照小鼠中，持续观察到脾集落形成单位（CFU-S）和粒-巨噬细胞集落形成单位（CFU-GM）增加。当将DDC处理动物的CFU-S和CFU-GM照射后存活率进行归一化处理以考虑到这种观察到的增加时，观察到保护因子范围为0.9至1.6。这些保护因子明显低于其他人报告的保护因子。在DDC处理后15至30分钟进行照射时观察到最大辐射防护作用；处理后1至2小时内CFU-GM出现最大增加。CFU-GM的增加类似于在用内毒素（一种非硫醇类辐射防护剂）处理的动物中观察到的增加。当用DDC攻击遗传上无法对内毒素作出反应的C3H/HeJ小鼠时，观察到CFU-GM平均增加1.7倍，这表明DDC的刺激作用不是由于内毒素污染。在照射前连续三天每天给予DDC，未观察到CFU-GM有很少或没有增加，也没有观察到辐射防护作用，这表明骨髓可能会对DDC产生耐受性。当在类似研究中测试WR-2721时，观察到剂量修正的辐射防护作用，对造血细胞没有明显的非特异性刺激作用。
