鼠伤寒沙门氏菌三肽通透酶（tpp）基因的遗传特征分析与分子克隆
Genetic characterization and molecular cloning of the tripeptide permease (tpp) genes of Salmonella typhimurium.
作者信息
Gibson M M, Price M, Higgins C F
出版信息
J Bacteriol. 1984 Oct;160(1):122-30. doi: 10.1128/jb.160.1.122-130.1984.
Abstract
Of the three bacterial peptide transport systems only one, the oligopeptide permease, has been characterized in any detail. We have now isolated Salmonella typhimurium mutants deficient in a second transport system, the tripeptide permease (Tpp), using the toxic peptide alafosfalin. Alafosfalin resistance mutations map at three loci, the gene encoding peptidase A (pepA) and two transport-defective loci, tppA and tppB. Locus tppA has been mapped to 74 min on the S. typhimurium chromosome, cotransducible with aroB, and is a positive regulator of tppB. Locus tppB maps at 27 min in the cotransduction gap between purB and pyrF. We cloned tppB, the structural locus for the tripeptide permease. Two simple methods are described for mapping the location of cloned DNA fragments on the chromosome of S. typhimurium.
摘要
在三种细菌肽转运系统中，只有一种，即寡肽通透酶，得到了较为详细的表征。我们现在利用有毒肽阿拉磷分离出了鼠伤寒沙门氏菌中缺乏第二种转运系统——三肽通透酶（Tpp）的突变体。阿拉磷抗性突变位于三个位点，即编码肽酶A的基因（pepA）和两个转运缺陷位点tppA和tppB。位点tppA已定位在鼠伤寒沙门氏菌染色体上74分钟处，与aroB共转导，是tppB的正调控因子。位点tppB位于purB和pyrF之间共转导间隙的27分钟处。我们克隆了tppB，即三肽通透酶的结构基因座。描述了两种简单的方法来确定克隆的DNA片段在鼠伤寒沙门氏菌染色体上的位置。
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本文引用的文献
1
Hfr formation directed by tn10.由 tn10 指导的高频转导形成。
Genetics. 1979 Apr;91(4):639-55. doi: 10.1093/genetics/91.4.639.
2
Toxicity of leucine-containing peptides in Escherichia coli caused by circumvention of leucine transport regulation.亮氨酸转运调节规避导致大肠杆菌中含亮氨酸肽的毒性。
J Bacteriol. 1981 May;146(2):676-83. doi: 10.1128/jb.146.2.676-683.1981.
3
Identification of a novel genetic element in Escherichia coli K-12.在大肠杆菌K-12中鉴定一种新型遗传元件。
J Bacteriol. 1980 Oct;144(1):312-21. doi: 10.1128/jb.144.1.312-321.1980.
4
Linkage map of Escherichia coli K-12, edition 6.大肠杆菌K-12连锁图谱，第6版。
Microbiol Rev. 1980 Mar;44(1):1-56. doi: 10.1128/mr.44.1.1-56.1980.
5
Anaerobic and leucine-dependent expression of a peptide transport gene in Salmonella typhimurium.鼠伤寒沙门氏菌中一种肽转运基因的厌氧和亮氨酸依赖性表达。
J Bacteriol. 1984 Oct;160(1):131-6. doi: 10.1128/jb.160.1.131-136.1984.
6
Phosphonopeptides as substrates for peptide transport systems and peptidases of Escherichia coli.膦肽作为大肠杆菌肽转运系统和肽酶的底物。
Antimicrob Agents Chemother. 1983 Oct;24(4):522-8. doi: 10.1128/AAC.24.4.522.
7
Isolation and characterization Salmonella typhimurium mutants lacking a tripeptidase (peptidase T).鼠伤寒沙门氏菌缺乏三肽酶（肽酶T）突变体的分离与鉴定
J Bacteriol. 1983 May;154(2):763-71. doi: 10.1128/jb.154.2.763-771.1983.
8
Genetic organization of the oligopeptide permease (opp) locus of Salmonella typhimurium and Escherichia coli.鼠伤寒沙门氏菌和大肠杆菌寡肽通透酶（opp）基因座的遗传组织
J Bacteriol. 1983 Mar;153(3):1548-51. doi: 10.1128/jb.153.3.1548-1551.1983.
9
Dipeptidyl carboxypeptidase-deficient mutants of Salmonella typhimurium.鼠伤寒沙门氏菌的二肽基羧肽酶缺陷型突变体。
J Bacteriol. 1983 Mar;153(3):1252-8. doi: 10.1128/jb.153.3.1252-1258.1983.
10
Linkage map of Salmonella typhimurium, Edition VI.鼠伤寒沙门氏菌连锁图谱，第六版。
Microbiol Rev. 1983 Sep;47(3):410-53. doi: 10.1128/mr.47.3.410-453.1983.
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