Gibson M M, Price M, Higgins C F
J Bacteriol. 1984 Oct;160(1):122-30. doi: 10.1128/jb.160.1.122-130.1984.
Of the three bacterial peptide transport systems only one, the oligopeptide permease, has been characterized in any detail. We have now isolated Salmonella typhimurium mutants deficient in a second transport system, the tripeptide permease (Tpp), using the toxic peptide alafosfalin. Alafosfalin resistance mutations map at three loci, the gene encoding peptidase A (pepA) and two transport-defective loci, tppA and tppB. Locus tppA has been mapped to 74 min on the S. typhimurium chromosome, cotransducible with aroB, and is a positive regulator of tppB. Locus tppB maps at 27 min in the cotransduction gap between purB and pyrF. We cloned tppB, the structural locus for the tripeptide permease. Two simple methods are described for mapping the location of cloned DNA fragments on the chromosome of S. typhimurium.
在三种细菌肽转运系统中，只有一种，即寡肽通透酶，得到了较为详细的表征。我们现在利用有毒肽阿拉磷分离出了鼠伤寒沙门氏菌中缺乏第二种转运系统——三肽通透酶（Tpp）的突变体。阿拉磷抗性突变位于三个位点，即编码肽酶A的基因（pepA）和两个转运缺陷位点tppA和tppB。位点tppA已定位在鼠伤寒沙门氏菌染色体上74分钟处，与aroB共转导，是tppB的正调控因子。位点tppB位于purB和pyrF之间共转导间隙的27分钟处。我们克隆了tppB，即三肽通透酶的结构基因座。描述了两种简单的方法来确定克隆的DNA片段在鼠伤寒沙门氏菌染色体上的位置。
