Menzel R, Roth J
J Bacteriol. 1980 Mar;141(3):1064-70. doi: 10.1128/jb.141.3.1064-1070.1980.
In this paper we demonstrate the existence of a second proline permease, gene proP, in Salmonella typhimurium. Uptake assays demonstrate that this second proline permease has 5 to 10% the uptake rate of the putP permease, the cell's major proline permease, when assayed at 20 microM proline. Genetic mapping by Hfr and P22-mediated genetic crosses placed the second proline permease gene at 92 min on the S. typhimurium genetic map, near the genes for melibiose utilization. F'-mediated complementation tests indicated that Escherichia coli also has the proP gene.
在本文中,我们证明了鼠伤寒沙门氏菌中存在第二种脯氨酸通透酶基因proP。摄取试验表明,当在20微摩尔脯氨酸浓度下进行测定时,这种第二种脯氨酸通透酶的摄取速率是细胞主要脯氨酸通透酶putP通透酶的5%至10%。通过高频重组(Hfr)和P22介导的遗传杂交进行的基因定位,将第二种脯氨酸通透酶基因定位于鼠伤寒沙门氏菌遗传图谱的92分钟处,靠近蜜二糖利用基因。F'介导的互补试验表明,大肠杆菌也具有proP基因。