Marrero R, Young F E, Yasbin R E
J Bacteriol. 1984 Oct;160(1):458-61. doi: 10.1128/jb.160.1.458-461.1984.
Plasmid pPL1010 is a 7.0-kilobase derivative of plasmid pUB110 that harbors the cohesive end site of the bacteriophage SP02 genome. Plasmid pPL1017 is a 6.8-kilobase derivative of plasmid pC194 that contains the immunity region of bacteriophage phi 105 and the cohesive end site of bacteriophage SP02. These plasmids are transducible by bacteriophage SP02 at a frequency of 10(-2) transductants per PFU among mutant derivatives of Bacillus subtilis 168 and have been transferred to other strains of B. subtilis and B. amyloliquefaciens by means of bacteriophage SP02-mediated transduction, with frequencies ranging from 10(-5) to 10(-7) transductants per PFU. The introduced plasmids were stably maintained in nearly all new hosts in the absence of selective pressure. An exception was found in B. subtilis DSM704, which also harbored three cryptic plasmids. Plasmids pPL1010 and pPL1017 were incompatible with a 7.9-kilobase replicon native to strain DSM704. Furthermore, plasmid pPL1017 was processed by strain DSM704 into a approximately 5.3-kilobase replicon that was compatible with the resident plasmid content of strain DSM704. The use of bacteriophage SP02-mediated plasmid transduction has allowed the identification of Bacillus strains that are susceptible to bacteriophage SP02-mediated genetic transfer but cannot support bacteriophage SP02 lytic infection.
质粒pPL1010是质粒pUB110的一个7.0千碱基的衍生物，它含有噬菌体SP02基因组的粘性末端位点。质粒pPL1017是质粒pC194的一个6.8千碱基的衍生物，它包含噬菌体phi 105的免疫区域和噬菌体SP02的粘性末端位点。这些质粒在枯草芽孢杆菌168的突变衍生物中可被噬菌体SP02以每噬菌斑形成单位（PFU）10⁻²转导子的频率转导，并且已经通过噬菌体SP02介导的转导转移到枯草芽孢杆菌和淀粉液化芽孢杆菌的其他菌株中，转导频率范围为每PFU 10⁻⁵至10⁻⁷转导子。在没有选择压力的情况下，导入的质粒在几乎所有新宿主中都能稳定维持。在枯草芽孢杆菌DSM704中发现了一个例外，该菌株还含有三个隐蔽质粒。质粒pPL1010和pPL1017与DSM704菌株天然存在的一个7.9千碱基的复制子不相容。此外，DSM704菌株将质粒pPL1017加工成了一个约5.3千碱基的复制子，该复制子与DSM704菌株的常驻质粒内容物相容。噬菌体SP02介导的质粒转导的应用使得能够鉴定出对噬菌体SP02介导的基因转移敏感但不能支持噬菌体SP02溶菌感染的芽孢杆菌菌株。
