Keggins K M, Lovett P S, Duvall E J
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1423-7. doi: 10.1073/pnas.75.3.1423.
Plasmid pUB110 (approximately 2.8 x 10(6) daltons), originally detected in Staphylococcus aureus, specifies resistance to neomycin and has been transformed into Bacillus subtilis 168, In B. subtilis, pUB110 is stably maintained at about 50 copies per chromosome and renders the host resistant to neomycin sulfate at 5 microgram/ml. pUB110 isolated from B. subtilis transforms Rec+ and recE4-containing strains of B. subtilis at frequencies greater than or equal to 10(3) transformants per microgram of plasmid. pUB110 was transferred by PBS1 or SP10 transduction from B. subtilis to strains of B. pumilus and B. licheniformis. pUB110 is compatible with each of four previously described Bacillus plasmids, including pPL576, pPL10, pPL7065, and pPL2. pUB110 contains a single EcoR1-sensitive site and was used as vector to clone DNA fragments that complement the trpC2 mutation in B. subtilis 168 from EcoR1 digests of the chromosome DNA isolated from B. pumilus strains NRRL B-3275 and NRS576, B. licheniformis strains 9945A and 749C, and B. subtilis 168. Genetic and physical properties of each of the constructed Trp derivatives of pUB110 are described.
质粒pUB110（约2.8×10⁶道尔顿）最初在金黄色葡萄球菌中被检测到，它赋予对新霉素的抗性，并且已被转化到枯草芽孢杆菌168中。在枯草芽孢杆菌中，pUB110以每条染色体约50个拷贝的数量稳定维持，并使宿主对5微克/毫升的硫酸新霉素具有抗性。从枯草芽孢杆菌中分离出的pUB110以大于或等于每微克质粒10³个转化子的频率转化枯草芽孢杆菌的Rec⁺和含recE4的菌株。pUB110通过PBS1或SP10转导从枯草芽孢杆菌转移到短小芽孢杆菌和地衣芽孢杆菌的菌株中。pUB110与先前描述的四种枯草芽孢杆菌质粒中的每一种都兼容，包括pPL576、pPL10、pPL7065和pPL2。pUB110含有一个单一的EcoR1敏感位点，并被用作载体来克隆来自短小芽孢杆菌菌株NRRL B - 3275和NRS576、地衣芽孢杆菌菌株9945A和749C以及枯草芽孢杆菌168的染色体DNA的EcoR1酶切片段，这些片段可互补枯草芽孢杆菌168中的trpC2突变。描述了构建的pUB110的每种色氨酸衍生物的遗传和物理特性。
