Costantino P, Spanò L, Pomponi M, Benvenuto E, Ancora G
J Mol Appl Genet. 1984;2(5):465-70.
Plants regenerated from tobacco hairy root callus cultures were fertilized by self-pollination, and healthy, morphologically normal R1 offspring were obtained. Three of these R1 seedlings were analyzed for the presence of T-DNA and synthesis of the T-DNA-specific compound agropine. All three R1 plants analyzed contained the same full-length T-DNA as the parental regenerant, while only two showed agropine synthesis.
从烟草毛状根愈伤组织培养物再生的植株通过自花授粉进行受精，获得了健康、形态正常的R1代后代。对其中三株R1代幼苗进行了T-DNA存在情况及T-DNA特异性化合物章鱼碱合成情况的分析。分析的所有三株R1代植株都含有与亲本再生植株相同的全长T-DNA，而只有两株表现出章鱼碱合成。
