Streiff F, Janot C, Briquel M E, Stoltz J F
Rev Fr Transfus Immunohematol. 1984 Jun;27(3):383-9. doi: 10.1016/s0338-4535(84)80179-8.
The authors describe the preparation of a first batch of intravenous cytomegalovirus (CMV) immune globulin at the Nancy Regional blood transfusion centre. Immune plasmas were selected from 3 640 healthy volunteer blood donors on the basis of CF antibody titers to CMV (Kolmer's method modified) of, at least, 1:8; plasmas from approximately 10% of the donors were therefore selected. The 68 liters of pooled immune plasma had à CF antibody titer of 1:16 (CMV antibody titers of 1: 10 000 and 1: 640 when tested in the ELISA assay and passive hemagglutination assay respectively). Intravenous immune globulin was produced from pooled plasma by Cohn fractionation and treatment with pepsin at pH 4; 4.8 liters of immune globulin were prepared and divided in 96 doses of 50 ml each. The final product was found to have a CMV antibody titer of 1: 32 (CF) 1: 50 000 (ELISA) or 1: 2 560 (passive hemagglutination). Recent reports on the preparation of CMV immune globulin are briefly reviewed.
作者描述了在南锡地区输血中心制备首批静脉注射巨细胞病毒（CMV）免疫球蛋白的过程。根据对CMV的CF抗体滴度（改良的科尔默方法）至少为1:8，从3640名健康志愿者献血者中筛选出免疫血浆；因此，大约10%的献血者的血浆被选中。68升混合免疫血浆的CF抗体滴度为1:16（在ELISA检测和被动血凝试验中检测时，CMV抗体滴度分别为1:10000和1:640）。静脉注射免疫球蛋白由混合血浆通过科恩分级分离法和在pH 4下用胃蛋白酶处理制备而成；制备了4.8升免疫球蛋白，并分成96剂，每剂50毫升。最终产品的CMV抗体滴度为1:32（CF）、1:50000（ELISA）或1:2560（被动血凝试验）。本文简要回顾了近期关于CMV免疫球蛋白制备的报道。
