MacLachlan N J, Schore C E, Osburn B I
Vet Immunol Immunopathol. 1984 Aug;7(1):11-8. doi: 10.1016/0165-2427(84)90023-0.
Bovine fetuses were inoculated with Mycobacterium bovis, bluetongue virus or placebo at approximately 125 days of gestation, and blastogenic responses of peripheral blood lymphocytes and lymph node cells were determined at various time intervals after inoculation. Lymphocytes from all fetuses were stimulated by phytohemagglutinin, concanavalin A, and pokeweed mitogen, and peripheral blood lymphocytes gave consistently greater stimulation indices than did prescapular lymph node cells. Bluetongue virus infection did not consistently suppress mitogen induced lymphocyte blastogenesis. Lymphocytes taken from fetuses at 20 or 50 days after Mycobacterium bovis inoculation were not stimulated by purified protein derivative (PPD), whereas lymphocytes taken from adult cattle at similar intervals after Mycobacterium bovis inoculation were stimulated by PPD. Although lymphocytes from bovine fetuses may be stimulated by mitogens, antigen specific blastogenesis to a known inducer of cellular immunity was not detected by 175 days of gestation.
在妊娠约125天时，给牛胎儿接种牛分枝杆菌、蓝舌病毒或安慰剂，并在接种后的不同时间间隔测定外周血淋巴细胞和淋巴结细胞的母细胞化反应。所有胎儿的淋巴细胞都受到植物血凝素、刀豆球蛋白A和商陆有丝分裂原的刺激，外周血淋巴细胞产生的刺激指数始终高于肩胛前淋巴结细胞。蓝舌病毒感染并未始终抑制有丝分裂原诱导的淋巴细胞母细胞化。在接种牛分枝杆菌后20或50天从胎儿采集的淋巴细胞未受到纯化蛋白衍生物（PPD）的刺激，而在接种牛分枝杆菌后相同间隔时间从成年牛采集的淋巴细胞则受到PPD的刺激。尽管牛胎儿的淋巴细胞可能受到有丝分裂原的刺激，但在妊娠175天时未检测到对已知细胞免疫诱导剂的抗原特异性母细胞化。
