Kettner M, Navarová J, Lebek G, Krcméry V
Zentralbl Bakteriol Mikrobiol Hyg A. 1984 Aug;257(3):372-82.
An in vitro evaluation of 32 gentamicin-resistant, Gram-negative bacteria from hygiene-centres in Berne, Switzerland and Bratislava, Czechoslovakia, revealed that 24 strains produced gentamicin-modifying enzymes. Occurrence of acetyltransferases (AAC) was confirmed in 16 strains, adenylyltransferase (AAD) /2''/ was observed in 13 strains while 5 of the gentamicin-resistant strains produced both enzymes. All Czechoslovak strains were amikacin-susceptible, in amikacin-resistant Pseudomonas aeruginosa strains from Switzerland the presence of AAC/6'/ was found. In the majority of gentamicin-resistant, netilmicin-susceptible strains the occurrence of AAD/2''/ was observed. Gentamicin-resistance in the modifying enzymes producing strains was due either to production of an acetyltransferase or the adenylyltransferase except Swiss strains of Serratia marcescens where a simultaneous production of both types of the enzymes was noted. Twenty eight strains produced an enzyme modifying aminoglycosides of any kind.
对来自瑞士伯尔尼和捷克斯洛伐克布拉迪斯拉发卫生中心的32株耐庆大霉素革兰氏阴性菌进行的体外评估显示，24株菌株产生了庆大霉素修饰酶。在16株菌株中证实存在乙酰转移酶（AAC），在13株菌株中观察到腺苷酰转移酶（AAD）/2''/，而5株耐庆大霉素菌株同时产生这两种酶。所有捷克斯洛伐克菌株对阿米卡星敏感，在来自瑞士的耐阿米卡星铜绿假单胞菌菌株中发现了AAC/6'/。在大多数耐庆大霉素、对奈替米星敏感的菌株中观察到AAD/2''/的存在。除瑞士粘质沙雷氏菌菌株同时产生两种类型的酶外，产生修饰酶的菌株中的庆大霉素耐药性要么是由于乙酰转移酶的产生，要么是由于腺苷酰转移酶的产生。28株菌株产生了修饰任何一种氨基糖苷类的酶。
