Inoue A, Aozasa K, Tsujimoto M, Tamai M, Chatani F, Ueno H
Acta Pathol Jpn. 1984 Jul;34(4):759-65. doi: 10.1111/j.1440-1827.1984.tb07604.x.
Malignant fibrous histiocytoma (MFH) shows a mixed proliferation of both fibroblastic and histiocytic cells. Because of their complex morphologic appearances, the nature of truly neoplastic cells in MFH has been controversial. In the present study, immunoperoxidase method (PAP method) was used to examine the intracytoplasmic lysozyme (LY), alpha-1-antitrypsin (A1AT), fibronectin (FN), and polyvalent immunoglobulin (PI) in the fibroblastic and histiocytic cells. Twenty-three cases with MFH were histologically divided into three groups; predominantly fibroblastic type (Group I; 5 cases), mixed fibroblastic and histiocytic type (Group II; 15 cases), and almost pure histiocytic type (Group III; 3 cases). Fibroblastic cells showed a strong positive reaction for LY and A1AT, suggesting the histiocytic nature, while the proliferating cells in Group II were more intensely stained by each of the antibodies than in Groups I and III. Enzyme histochemical examinations on fresh materials were available in 3 cases. These findings suggest that proliferating cells in MFH possess a histiocyte nature.
恶性纤维组织细胞瘤（MFH）表现为成纤维细胞和组织细胞的混合性增殖。由于其复杂的形态学表现，MFH中真正肿瘤细胞的性质一直存在争议。在本研究中，采用免疫过氧化物酶法（PAP法）检测成纤维细胞和组织细胞胞质内的溶菌酶（LY）、α1抗胰蛋白酶（A1AT）、纤连蛋白（FN）和多价免疫球蛋白（PI）。23例MFH组织学上分为三组：以成纤维细胞为主型（I组；5例）、成纤维细胞和组织细胞混合型（II组；15例）和几乎为纯组织细胞型（III组；3例）。成纤维细胞对LY和A1AT呈强阳性反应，提示其具有组织细胞性质，而II组中增殖细胞被每种抗体染色的程度均比I组和III组更强烈。3例新鲜标本进行了酶组织化学检查。这些结果提示MFH中的增殖细胞具有组织细胞性质。
