Dayer J M, Zavadil-Grob C, Ucla C, Mach B
Eur J Immunol. 1984 Oct;14(10):898-901. doi: 10.1002/eji.1830141007.
Human blood peripheral monocyte/macrophages release in culture a mononuclear cell factor (MCF) which stimulates the production of collagenase and prostaglandin E2 by human rheumatoid synovial cells and dermal fibroblasts. These two products play a role in connective tissue destruction. MCF has an apparent molecular weight of approximately 15 000 and is biologically and biochemically indistinguishable from interleukin 1. MCF therefore belongs to the well-documented nonimmune biological activities attributed to interleukin 1. Studies on the mechanisms of production and action of such monokine(s) have been difficult in view of the minute quantities produced by freshly isolated cells or from human monocytic lines. Starting from lectin-stimulated human blood mononuclear cells, we have isolated poly(A)+ RNA and studied its translation following microinjection into Xenopus laevis oocytes. The mRNA translation products stimulated collagenase and prostaglandin E2 production in human rheumatoid synovial cells and dermal fibroblasts. The size of MCF-mRNA was estimated to be 10 S. The mRNA of a member of the interleukin 1 family can now be studied in a system based on a specific and direct relevant biological assay and eventually compared with those of other monokines.
人外周血单核细胞/巨噬细胞在培养中释放一种单核细胞因子（MCF），该因子可刺激人类风湿性滑膜细胞和真皮成纤维细胞产生胶原酶和前列腺素E2。这两种产物在结缔组织破坏中起作用。MCF的表观分子量约为15000，在生物学和生物化学上与白细胞介素1无法区分。因此，MCF属于已充分证明的归因于白细胞介素1的非免疫生物学活性。鉴于新鲜分离的细胞或人单核细胞系产生的此类单核因子数量极少，对其产生和作用机制的研究一直很困难。从凝集素刺激的人血单核细胞开始，我们分离了聚腺苷酸加尾RNA（poly(A)+ RNA），并在显微注射到非洲爪蟾卵母细胞后研究了其翻译情况。mRNA翻译产物刺激了人类风湿性滑膜细胞和真皮成纤维细胞中胶原酶和前列腺素E2的产生。MCF-mRNA的大小估计为10S。现在可以在基于特定且直接相关生物学测定的系统中研究白细胞介素1家族成员的mRNA，并最终与其他单核因子的mRNA进行比较。
