Dambinova S A, Besedin V I, Gorodinskiĭ A I, Margulis M N, Pavlova O I
Fiziol Zh SSSR Im I M Sechenova. 1984 Jul;70(7):952-60.
The transport and selective functions of the glutamate-binding proteins of the rat brain cortex synaptic membranes, were studied. The data on kinetics of absorption of the ions 22Na+, 86Rb+ and 45Ca++ by the membrane vesicles and liposomes containing receptor proteins, are presented. The specific features of the c. n. s. glutamate receptors functioning in the hybrid cells of the neuroblastoma N18Tg2a, are revealed. A selective activation of transport of the 22Na+ ions was found in this kind of model systems in presence of physiological concentrations of L-glutamate. The modelling of the glutamate receptors function depended on composition of lipids, presence of the endogenous peptide agent inhibiting binding of the H3-L-glutamate, and on the degree of the neuroblastoma differentiation. Monoclonal antibodies obtained for the receptor's recognizing areas blocked the functions of the glutamate-binding proteins in all the model systems.
对大鼠大脑皮层突触膜谷氨酸结合蛋白的转运和选择功能进行了研究。给出了含有受体蛋白的膜囊泡和脂质体对离子(^{22}Na^+)、(^{86}Rb^+)和(^{45}Ca^{++})吸收动力学的数据。揭示了神经母细胞瘤N18Tg2a杂交细胞中中枢神经系统谷氨酸受体发挥功能的具体特征。在这种模型系统中，当存在生理浓度的L-谷氨酸时，发现(^{22}Na^+)离子的转运被选择性激活。谷氨酸受体功能的模拟取决于脂质组成、抑制(H3-L)-谷氨酸结合的内源性肽试剂的存在以及神经母细胞瘤的分化程度。针对受体识别区域获得的单克隆抗体在所有模型系统中均阻断了谷氨酸结合蛋白的功能。
