Bobryshev Iu V, Orlova E A, Balabanova Iu V, Kuznetsov A S, Smirnova T M
Tsitologiia. 1989 Feb;31(2):176-81.
The aim of the study was to analyse the distribution and localization of glutamate receptors in the cultured cells of the neonatal rat motor cortex, using immunoelectron microscopic technique, and monoclonal antibodies preliminary labeled with colloidal gold. Monoclonal antibodies against glutamate-binding proteins of the adult rat cerebral cortex were produced by means of hybridization of immune splenocytes with plasmocytoma cells. It was found that monoclonal antibodies labeled with colloidal gold could reveal selectively the localization of glutamate receptors on the membranes of neurons. Glutamate receptors were detected on differentiating neuron membranes only, being absent from the glia cell surface.
本研究旨在运用免疫电子显微镜技术及预先用胶体金标记的单克隆抗体，分析新生大鼠运动皮质培养细胞中谷氨酸受体的分布及定位。通过免疫脾细胞与浆细胞瘤细胞杂交的方法，制备了针对成年大鼠大脑皮质谷氨酸结合蛋白的单克隆抗体。结果发现，用胶体金标记的单克隆抗体能够选择性地揭示谷氨酸受体在神经元膜上的定位。谷氨酸受体仅在分化中的神经元膜上被检测到，胶质细胞表面则未发现。
