Institute of Applied Cell Culture, D-81669, München, Germany.
Cytotechnology. 1996 Jan;21(3):183-93. doi: 10.1007/BF00365341.
This article describes the current status in the development of human monoclonal antibodies. Over the last ten years a lot of information about the human immune system has emerged. Combining these with the many new (bio-)technologies it is plausible that the long awaited breakthrough of this technology is close. This paper focuses on the "classical" cell-biological methods of achieving stable, antibody-producing human cell lines via cell fusion methods or virus derived transformations of human B-lymphocytes, as well as genetic engineering methods e.g. DNA libraries or phage display technology. The available in vitro immunization methods are critically reviewed and their impact on this topic is discussed. Therapeutic applications for cancer treatment or passive immunization against infectious diseases with antibodies derived by both ways are also reviewed.
本文描述了人类单克隆抗体的发展现状。在过去的十年中，人们对人类免疫系统有了很多了解。将这些知识与许多新的（生物）技术相结合，这项技术的长期突破似乎即将实现。本文重点介绍了通过细胞融合方法或病毒转化人 B 淋巴细胞来获得稳定产生抗体的人细胞系的“经典”细胞生物学方法，以及遗传工程方法，例如 DNA 文库或噬菌体展示技术。本文还批判性地回顾了现有的体外免疫方法，并讨论了它们对这一主题的影响。此外，还综述了通过这两种方法获得的抗体在癌症治疗或传染病被动免疫中的治疗应用。
