Spear G T, Teodorescu M
J Immunol Methods. 1984 Oct 26;73(2):321-7. doi: 10.1016/0022-1759(84)90407-1.
Addition of 50 micrograms LPS/ml to as little as 5 pg of LPS/ml to the allogeneic human mixed leukocyte reaction (MLR) enhanced [3H]thymidine incorporation 2-4-fold. The same concentrations of LPS had no effect on thymidine incorporation in cultures without the allogeneic cells. The Limulus amebocyte lysate (LAL) test we used was sensitive only to 15 pg LPS/ml indicating that the MLR is more sensitive to LPS than the LAL test. A commercially available RPMI 1640 culture medium used by many investigators was found to contain concentrations of LPS that would significantly enhance the MLR. Polymyxin B blocked the MLR-enhancing effect of LPS while having no inhibitory or stimulating effect itself. We concluded that low concentrations of LPS are helpful in revealing MLR incompatibilities by enhancing the lymphocyte responses and that the addition of polymyxin B is essential in the study of factors affecting the MLR by eliminating any LPS contribution.
在同种异体人混合淋巴细胞反应（MLR）中，向低至5皮克/毫升的脂多糖（LPS）中添加50微克/毫升的LPS，可使[3H]胸腺嘧啶核苷掺入量增加2至4倍。相同浓度的LPS对不含同种异体细胞的培养物中的胸腺嘧啶核苷掺入没有影响。我们使用的鲎试剂（LAL）检测仅对15皮克/毫升的LPS敏感，这表明MLR对LPS比LAL检测更敏感。许多研究者使用的市售RPMI 1640培养基被发现含有能显著增强MLR的LPS浓度。多粘菌素B可阻断LPS对MLR的增强作用，而其本身没有抑制或刺激作用。我们得出结论，低浓度的LPS通过增强淋巴细胞反应有助于揭示MLR不相容性，并且在研究影响MLR的因素时，添加多粘菌素B对于消除任何LPS的影响至关重要。
