Li P, Bellett A J, Parish C R
J Gen Virol. 1984 Oct;65 ( Pt 10):1803-15. doi: 10.1099/0022-1317-65-10-1803.
Chick embryo lethal orphan (CELO) virus (fowl adenovirus type 1) contains at least 14 structural proteins with polypeptide molecular weights ranging from 100K to about 6K. A nomenclature of the CELO virion polypeptides is presented and the molar proportion of each polypeptide has been estimated. The CELO virus pentons were specifically released from the virion by dialysis against borate-based calcium-magnesium saline. The penton base (polypeptide III, mol. wt. 92K) and two fibres were separated, characterized and their polypeptides were correlated with their morphological positions in the virion. Peptide mapping suggested that the long fibre (polypeptide IV, mol. wt. 65K), and the short fibre (polypeptide VII, mol. wt. 44.5K) were not related in their primary sequences and are therefore probably encoded by separate genes. The time course of synthesis of the CELO virion polypeptides indicated that, like their mammalian adenovirus counterparts, they are synthesized late (after viral DNA replication).
鸡胚致死孤儿（CELO）病毒（禽腺病毒1型）含有至少14种结构蛋白，其多肽分子量范围从100K到约6K。本文给出了CELO病毒粒子多肽的命名法，并估算了每种多肽的摩尔比例。通过用硼酸盐基钙镁盐水透析，CELO病毒的五邻体从病毒粒子中特异性释放出来。分离并鉴定了五邻体基部（多肽III，分子量92K）和两根纤维，并将它们的多肽与其在病毒粒子中的形态位置相关联。肽图谱分析表明，长纤维（多肽IV，分子量65K）和短纤维（多肽VII，分子量44.5K）在其一级序列上不相关，因此可能由不同基因编码。CELO病毒粒子多肽的合成时间进程表明，与它们的哺乳动物腺病毒对应物一样，它们在晚期（病毒DNA复制后）合成。
