Purchio A F, Larson R, Torborg L L, Collett M S
J Virol. 1984 Dec;52(3):973-5. doi: 10.1128/JVI.52.3.973-975.1984.
Bovine viral diarrhea virus RNA was translated in a reticulocyte cell-free protein synthesizing system. The purified, 8.2-kilobase, virus-specific RNA species was unable to serve an an efficient message unless it was denatured immediately before translation. In this case, several polypeptides, ranging in molecular weight from 50,000 to 150,000 and most of which were immunoprecipitated by bovine viral diarrhea virus-specific antiserum, were synthesized in vitro. When polyribosomes were used to program cell-free synthesis, mature viral 80,000- and 115,000-molecular-weight proteins were detected; no precursor to the viral 55,000-molecular-weight glycoprotein was noted. The implications of these results with respect to virus-specific protein synthesis are discussed.
牛病毒性腹泻病毒RNA在网织红细胞无细胞蛋白质合成系统中进行翻译。纯化的8.2千碱基病毒特异性RNA种类不能作为有效的信使，除非在翻译前立即变性。在这种情况下，体外合成了几种分子量在50,000至150,000之间的多肽，其中大部分被牛病毒性腹泻病毒特异性抗血清免疫沉淀。当使用多核糖体来编程无细胞合成时，检测到成熟的病毒80,000和115,000分子量的蛋白质；未发现病毒55,000分子量糖蛋白的前体。讨论了这些结果对病毒特异性蛋白质合成的影响。
