Bankier A T, Deininger P L, Farrell P J, Barrell B G
Mol Biol Med. 1983 Jul;1(1):21-45.
In order to provide a framework for understanding the molecular biology of Epstein-Barr virus (EBV), we are determining the DNA sequence of the virus and studying the organization of genes on the viral genome. In this paper we report the DNA sequence of the EcoRI C fragment of the B95-8 strain of EBV. The large (approximately 13.6 kb) deletion in this strain has been located by comparison with the DNA sequence of EBV isolated from Raji cells. The sequence has been analysed for possible protein coding regions and transcriptional control sites. At least eight large open reading frames are found, some of them associated with canonical promoter and polyadenylation sequences. The sequences of some of the encoded proteins suggest that they are membrane proteins. It is known that antibodies to major membrane glycoproteins of EBV can neutralize infection in tissue culture. A possible relationship between some of the encoded proteins and the major membrane glycoproteins of the virus is discussed.
为了提供一个理解爱泼斯坦-巴尔病毒（EBV）分子生物学的框架，我们正在确定该病毒的DNA序列，并研究病毒基因组上基因的组织方式。在本文中，我们报告了EBV B95-8株EcoRI C片段的DNA序列。通过与从Raji细胞分离的EBV的DNA序列比较，确定了该株中的大片段（约13.6 kb）缺失。对该序列进行了分析，以寻找可能的蛋白质编码区和转录控制位点。发现了至少八个大的开放阅读框，其中一些与典型的启动子和聚腺苷酸化序列相关。一些编码蛋白的序列表明它们是膜蛋白。已知针对EBV主要膜糖蛋白的抗体可在组织培养中中和感染。文中讨论了一些编码蛋白与病毒主要膜糖蛋白之间可能的关系。
