Bankier A T, Deininger P L, Satchwell S C, Baer R, Farrell P J, Barrell B G
Mol Biol Med. 1983 Nov;1(4):425-45.
An analysis of the approximately 12,440 base-pair sequence of the EcoRI Dhet fragment isolated from the circular episomal form of the B95-8 strain of Epstein-Barr virus is presented. This fragment contains the covalently joined ends of the intracellular episomal form of the molecule. In the viral capsid the DNA is linear and the joining is mediated via the terminal repeated DNA. Four copies of tandem repeated DNA were present in this clone, three with a repeat size of 538 and one of 523. The positions of a number of possible protein coding regions and transcription signals are discussed. In particular a possible spliced coding region for an approximately 45,000 Mr protein expressed in latently infected transformed cells is proposed. The predicted protein sequence contains hydrophobic regions separated by charged amino acids reminiscent of a membrane protein.
本文对从爱泼斯坦 - 巴尔病毒B95 - 8株的环状附加体形式中分离出的EcoRI Dhet片段约12440个碱基对的序列进行了分析。该片段包含分子细胞内附加体形式的共价连接末端。在病毒衣壳中，DNA是线性的，连接是通过末端重复DNA介导的。该克隆中存在四个串联重复DNA拷贝，三个重复大小为538，一个为523。讨论了一些可能的蛋白质编码区和转录信号的位置。特别提出了一个可能在潜伏感染的转化细胞中表达的约45000 Mr蛋白质的剪接编码区。预测的蛋白质序列包含由带电荷氨基酸分隔的疏水区域，让人联想到膜蛋白。
