Pichersky E, Gottlieb L D, Hess J F
Mol Gen Genet. 1984;195(1-2):314-20. doi: 10.1007/BF00332765.
We report here the complete nucleotide sequence of the E. coli triose phosphate isomerase gene. The gene encodes a polypeptide of 255 amino acids which is approximately 46% homologous to eukaryotic triose phosphate isomerases, and approximately 38% homologous to the enzyme from a thermophilic bacterium, Bacillus stearothermophilus. The nucleotide sequence is 55% homologous to that of the corresponding gene in the yeast Saccharomyces cerevisiae. To our knowledge, this is the first report of the sequence of a gene coding a glycolytic enzyme from a prokaryotic organism.
我们在此报告大肠杆菌磷酸丙糖异构酶基因的完整核苷酸序列。该基因编码一个由255个氨基酸组成的多肽，它与真核生物的磷酸丙糖异构酶约有46%的同源性，与嗜热细菌嗜热脂肪芽孢杆菌的该酶约有38%的同源性。其核苷酸序列与酿酒酵母中相应基因的序列有55%的同源性。据我们所知，这是关于原核生物中编码糖酵解酶的基因序列的首次报道。
