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参与核苷补救途径的梨支原体基因的鉴定。

Identification of Mycoplasma pirum genes involved in the salvage pathways for nucleosides.

作者信息

Tham T N, Ferris S, Kovacic R, Montagnier L, Blanchard A

机构信息

Departement du SIDA et des rétrovirus, Oncologie Virale, Institut Pasteur, Paris, France.

出版信息

J Bacteriol. 1993 Aug;175(16):5281-5. doi: 10.1128/jb.175.16.5281-5285.1993.

DOI:10.1128/jb.175.16.5281-5285.1993
PMID:8349569
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC204999/
Abstract

Genes encoding enzymes involved in the salvage pathway for nucleosides have been cloned and sequenced from the mollicute Mycoplasma pirum. One of them, encoding deoxyriboaldolase, was functionally identified by complementation of an Escherichia coli mutant. These genes are clustered, suggesting an operon organization, and they are immediately followed by the putative gene for the triose phosphate isomerase, an enzyme used during glycolysis.

摘要

编码参与核苷补救途径的酶的基因已从柔膜菌属的梨支原体中克隆并测序。其中一个编码脱氧核糖醛缩酶的基因通过对大肠杆菌突变体的互补作用进行了功能鉴定。这些基因成簇排列,表明存在操纵子结构,并且在它们之后紧接着是磷酸丙糖异构酶的推定基因,磷酸丙糖异构酶是糖酵解过程中使用的一种酶。

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本文引用的文献

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Primary structure of triosephosphate isomerase from Bacillus stearothermophilus.嗜热脂肪芽孢杆菌磷酸丙糖异构酶的一级结构
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Nucleotide sequence of the triose phosphate isomerase gene of Escherichia coli.大肠杆菌磷酸丙糖异构酶基因的核苷酸序列
Mol Gen Genet. 1984;195(1-2):314-20. doi: 10.1007/BF00332765.
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The internal regulated promoter of the deo operon of Escherichia coli K-12.大肠杆菌K-12中deo操纵子的内部调控启动子。
Nucleic Acids Res. 1984 Jul 11;12(13):5211-24. doi: 10.1093/nar/12.13.5211.
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Analysis of the terminator region after the deoCABD operon of Escherichia coli K-12 using a new class of single copy number operon-fusion vectors.利用一类新型单拷贝数操纵子融合载体对大肠杆菌K-12的deoCABD操纵子后的终止子区域进行分析。
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Assay for detection of adenosine phosphorylase from mycoplasmas.支原体中腺苷磷酸化酶的检测试验
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