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转染DNA的短期高效表达。

Short-term, high-efficiency expression of transfected DNA.

作者信息

Sussman D J, Milman G

出版信息

Mol Cell Biol. 1984 Aug;4(8):1641-3. doi: 10.1128/mcb.4.8.1641-1643.1984.

DOI:10.1128/mcb.4.8.1641-1643.1984
PMID:6092925
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC368960/
Abstract

We have achieved high-efficiency uptake and expression of foreign DNA in mouse Ltk- cells by modifying the DEAE-dextran-mediated transfection method of McCutchan and Pagano (J. Natl. Cancer Inst. 42:351-357, 1968) to include an initial incubation at elevated pH followed by a shock treatment with dimethyl sulfoxide. Up to 80% of mouse Ltk- cells transfected with the herpes simplex virus thymidine kinase gene expressed thymidine kinase as measured by autoradiography.

摘要

我们通过改进麦卡琴和帕加诺（《国家癌症研究所杂志》42:351 - 357，1968年）的DEAE - 葡聚糖介导的转染方法，实现了外源DNA在小鼠Ltk⁻细胞中的高效摄取和表达。改进后的方法包括在较高pH值下进行初始孵育，随后用二甲基亚砜进行休克处理。通过放射自显影法测定，用单纯疱疹病毒胸苷激酶基因转染的小鼠Ltk⁻细胞中，高达80%表达了胸苷激酶。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f0d/368960/c6f038f9888e/molcellb00150-0222-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f0d/368960/c6f038f9888e/molcellb00150-0222-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f0d/368960/c6f038f9888e/molcellb00150-0222-a.jpg

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