鸡肠道中DNA结合型和非DNA结合型1,25-二羟基维生素D受体的亚细胞分布
Subcellular distribution of DNA-binding and non-DNA-binding 1,25-dihydroxyvitamin D receptors in chicken intestine.
作者信息
Nakada M, Simpson R U, DeLuca H F
出版信息
Proc Natl Acad Sci U S A. 1984 Nov;81(21):6711-3. doi: 10.1073/pnas.81.21.6711.
A comparison of 1,25-dihydroxyvitamin D3 receptor concentration and equilibrium dissociation constants of whole tissue, nuclear, and cytosol extracts of vitamin D-deficient chicken intestine has been carried out. The extracts had the following order of receptor concentration: nuclei greater than whole tissue greater than cytosol. The receptors in each preparation had identical equilibrium dissociation constants (Kd) for 1,25-dihydroxyvitamin D3. However, the receptor in the cytosol fraction did not bind to DNA-cellulose, while a large fraction of the receptor population in crude nuclear extract and whole tissue extract bound to DNA-cellulose and could be eluted with 0.22 M KCl, suggesting that the cytosolic form of the receptor does not possess a DNA binding site.
对维生素D缺乏的鸡肠道全组织、细胞核和细胞质提取物的1,25 - 二羟基维生素D3受体浓度及平衡解离常数进行了比较。提取物的受体浓度顺序如下：细胞核大于全组织大于细胞质。每种制剂中的受体对1,25 - 二羟基维生素D3具有相同的平衡解离常数（Kd）。然而，细胞质部分的受体不与DNA - 纤维素结合，而粗核提取物和全组织提取物中的大部分受体群体与DNA - 纤维素结合，并能用0.22 M KCl洗脱，这表明受体的细胞质形式不具有DNA结合位点。
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