Adams G A, Feuerstein I A
Thromb Haemost. 1984 Aug 31;52(1):45-9.
We have studied the accumulation of washed platelets on collagen-coated glass from flowing platelet-red blood cell suspensions in the presence and absence of drugs. Glass tubes were 10 cm long and the flow rate was 1 ml/min, 80 s-1. For all experiments, platelet accumulation was greatest near the tube's inlet with a continuous decrease to the exit. A common feature, of those drug treatments which lead to reduced accumulation at the inlet, was an increase in outlet accumulation when compared with outlet control values. Platelet-collagen adhesion resulted in maximal release of 3H-serotonin in the presence of agents that prevent platelet aggregation on collagen. Only drug treatment known to raise cAMP levels (PGE1 and dipyridamole) or prevent the formation of prostaglandins and thromboxanes (sulfinpyrazone, indomethacin and ASA) were found to inhibit aggregate growth. Platelet aggregation on collagen in the absence of thrombin likely proceeds through the liberation of prostaglandins and thromboxanes from surface-bound platelets into the flow where they may stimulate flow-born cells. An alternate hypothesis is that such treatments affect the release of alpha-granule components necessary for aggregation.
我们研究了在有药物和无药物存在的情况下，从流动的血小板 - 红细胞悬液中，洗涤后的血小板在胶原包被玻璃上的积聚情况。玻璃管长10厘米，流速为1毫升/分钟，80秒^-1。对于所有实验，血小板积聚在管入口处最大，并向出口处持续减少。那些导致入口处积聚减少的药物处理的一个共同特征是，与出口对照值相比，出口处积聚增加。在存在防止血小板在胶原上聚集的试剂的情况下，血小板 - 胶原粘附导致3H - 血清素的最大释放。仅发现已知可提高cAMP水平的药物处理（前列腺素E1和双嘧达莫）或防止前列腺素和血栓素形成的药物处理（磺吡酮、吲哚美辛和阿司匹林）可抑制聚集体生长。在没有凝血酶的情况下，血小板在胶原上的聚集可能通过前列腺素和血栓素从表面结合的血小板释放到流动中进行，在流动中它们可能刺激流动中的细胞。另一种假设是，此类处理会影响聚集所需的α - 颗粒成分的释放。
