Rice N R, Stephens R M, Couez D, Deschamps J, Kettmann R, Burny A, Gilden R V
Virology. 1984 Oct 15;138(1):82-93. doi: 10.1016/0042-6822(84)90149-1.
The env gene of a bovine leukemia virus (BLV) tumor-derived proviral DNA clone has been located by comparison of the translated DNA sequence with amino acid sequence data on purified gp60 and p30env (A. M. Schultz, T. D. Copeland, and S. Oroszlan (1984) Virology 135, 417-427). There is a continuous open reading frame from the N terminus of gp60 for 1446 nucleotides; gp60 is predicted to contain 268 amino acids and p30env, 214. The predicted p30env shows structural features typical of type C viral transmembrane proteins. It is also clearly related to that of the human T-cell leukemia virus (HTLV), as predicted from the DNA sequence of Seiki et al. (M. Seiki, S. Hattori, Y. Hirayama, and M. Yoshida (1983) Proc. Natl. Acad. Sci. USA 80, 3618-3622) The two proteins show 36% identities in their amino acid sequence, in an alignment requiring six gaps. More distant relatedness is also seen between BLV p30env and both murine leukemia virus p15E and Rous sarcoma virus gp36. The gp60s of BLV and HTLV are more distantly related than their p30envs, but their homology is nonetheless statistically significant. Between the presumptive terminator of the env gene and the beginning of the 3'-long terminal repeat is a region of 1817 base pairs of unknown function. Just as in the HTLV post-envelope sequence, there are at least two reading frames which are open for a significant fraction of this region. In neither the tumor-derived clone nor a clone from a virus-producing cell line, however, is there a continuous open reading frame throughout the region. Comparison of the BLV and HTLV sequences within the post-envelope region revealed a very limited but possibly significant similarity.
通过将翻译后的DNA序列与纯化的gp60和p30env的氨基酸序列数据进行比较，确定了牛白血病病毒（BLV）肿瘤衍生前病毒DNA克隆的env基因位置（A.M.舒尔茨、T.D.科普兰和S.奥罗斯兰（1984年），《病毒学》135卷，第417 - 427页）。从gp60的N端开始有一个1446个核苷酸的连续开放阅读框；预计gp60含有268个氨基酸，p30env含有214个氨基酸。预测的p30env显示出C型病毒跨膜蛋白的典型结构特征。正如从Seiki等人的DNA序列所预测的那样（M.Seiki、S.服部、Y.平山和M.吉田（1983年），《美国国家科学院院刊》80卷，第3618 - 3622页），它也与人类T细胞白血病病毒（HTLV）的p30env明显相关。在需要六个空位的比对中，这两种蛋白质的氨基酸序列有36%的同一性。在BLV p30env与小鼠白血病病毒p15E和劳氏肉瘤病毒gp36之间也观察到更远的亲缘关系。BLV和HTLV的gp60之间的亲缘关系比它们的p30env更远，但它们的同源性在统计学上仍然是显著的。在env基因的推定终止子与3' - 长末端重复序列的起始之间是一个1817个碱基对的功能未知区域。就像在HTLV包膜后序列中一样，在该区域的很大一部分至少有两个开放阅读框。然而，在肿瘤衍生克隆和病毒产生细胞系的克隆中，整个区域都没有连续的开放阅读框。比较BLV和HTLV包膜后区域的序列发现了非常有限但可能有意义的相似性。
