Rice N R, Simek S L, Dubois G C, Showalter S D, Gilden R V, Stephens R M
J Virol. 1987 May;61(5):1577-85. doi: 10.1128/JVI.61.5.1577-1585.1987.
Bovine leukemia virus, like its closest relatives the human T-cell leukemia virus types I and II, contains a 1.8-kilobase X region between the env gene and the 3' long terminal repeat. In this communication, we report the detection and characterization of a subgenomic mRNA from which this X region is presumably translated. This mRNA was produced by a complex splicing mechanism which resulted in juxtaposition of the 5' end of the env gene and the two overlapping X-region open reading frames. Translation of this mRNA could yield at least two distinct proteins depending on which initiation codon is used. Detection of the protein encoded by the BLV X-region long open reading frame has been reported (N. Sagata, J. Tsuzuku-Kawamura, M. Nagayoshi-Aida, F. Shimizu, K.-I. Imagawa, and Y. Ikawa, Proc. Natl. Acad. Sci. USA 82:7879-7883, 1985). Using synthetic peptide antisera, we detected a protein encoded by the short open reading frame in virus-infected cells. The protein migrated in sodium dodecyl sulfate-polyacrylamide gels with an apparent molecular weight of 19,000. It is a nuclear phosphoprotein.
牛白血病病毒与其亲缘关系最近的人类T细胞白血病病毒I型和II型一样，在env基因和3'长末端重复序列之间含有一个1.8千碱基的X区域。在本通讯中，我们报告了一种亚基因组mRNA的检测和特性，该X区域可能是由这种mRNA翻译而来的。这种mRNA是通过一种复杂的剪接机制产生的，该机制导致env基因的5'端与两个重叠的X区域开放阅读框并列。根据使用的起始密码子不同，这种mRNA的翻译可以产生至少两种不同的蛋白质。已经报道了对由BLV X区域长开放阅读框编码的蛋白质的检测（N. Sagata、J. Tsuzuku-Kawamura、M. Nagayoshi-Aida、F. Shimizu、K.-I. Imagawa和Y. Ikawa，《美国国家科学院院刊》82:7879 - 7883，1985年）。我们使用合成肽抗血清在病毒感染的细胞中检测到了由短开放阅读框编码的一种蛋白质。该蛋白质在十二烷基硫酸钠 - 聚丙烯酰胺凝胶中迁移，表观分子量为19,000。它是一种核磷蛋白。
