Read G S, Sharp J A, Summers W C
Virology. 1984 Oct 30;138(2):368-72. doi: 10.1016/0042-6822(84)90363-5.
The sites of in vitro transcription initiation on the BamHI Q fragment of herpes simplex virus DNA have been compared with the sites of 5' ends of RNAs made in vivo after virus infection. S1-nuclease protection analysis of these RNAs shows that there are in vivo counterparts for each of the five previously identified in vitro transcripts. The whole-cell-extract RNA polymerase II transcription system faithfully initiates RNAs predominantly at bona fide in vivo start sites and gives few, if any, false positive start sites. Further, antiparallel, self-complementary transcripts from the BamHI Q fragment were observed in the coding region of the HSV thymidine kinase gene.
已将单纯疱疹病毒DNA的BamHI Q片段上的体外转录起始位点与病毒感染后体内合成的RNA的5'末端位点进行了比较。对这些RNA的S1核酸酶保护分析表明，先前鉴定的五个体外转录本中的每一个在体内都有对应物。全细胞提取物RNA聚合酶II转录系统忠实地主要在真正的体内起始位点起始RNA，并且几乎没有（如果有的话）假阳性起始位点。此外，在HSV胸苷激酶基因的编码区观察到来自BamHI Q片段的反平行、自我互补转录本。
