Beattie M A, Winder W W
Am J Physiol. 1984 Nov;247(5 Pt 2):R780-5. doi: 10.1152/ajpregu.1984.247.5.R780.
We have examined the roles of liver glycogen and malonyl coenzyme A (CoA) in determining the degree of postexercise ketosis in endurance-trained and nontrained rats. Three groups of rats were run on a treadmill for 90 min: trained (2 h/day, 6 wk) and food restricted to 5.5 g/100 g body wt the night before the 90-min exercise bout (group 1), nontrained fed ad libitum (group 2), and nontrained food restricted (same as trained) (group 3). Liver glycogen was 34 +/- 5, 24 +/- 2, and 7 +/- 2 mg/g in groups 1, 2, and 3, respectively, at the end of exercise. At the end of exercise and during the postexercise period the blood 3-hydroxybutyrate concentration in group 3 was significantly higher than in groups 1 or 2. No difference was observed between groups 1 and 2 in blood 3-hydroxybutyrate. Hepatic malonyl CoA was decreased to the same extent in all rats during exercise but remained depressed only in the glycogen-depleted group 3 rats in the postexercise period. These data suggest that the differences in degree of ketonemia in the postexercise period (but not during exercise) were due to lower hepatic malonyl CoA in group 3 rats.
我们研究了肝糖原和丙二酰辅酶A（CoA）在耐力训练和未训练大鼠运动后酮血症程度决定中的作用。三组大鼠在跑步机上跑90分钟：训练组（每天2小时，共6周），并在90分钟运动前一晚将食物限制为5.5克/100克体重（第1组）；未训练组自由进食（第2组）；未训练组食物限制（与训练组相同）（第3组）。运动结束时，第1、2、3组的肝糖原分别为34±5、24±2和7±2毫克/克。运动结束时及运动后期间，第3组的血液3-羟基丁酸浓度显著高于第1组或第2组。第1组和第2组的血液3-羟基丁酸无差异。运动期间所有大鼠的肝丙二酰辅酶A均下降至相同程度，但运动后期间仅糖原耗尽的第3组大鼠的肝丙二酰辅酶A仍处于较低水平。这些数据表明，运动后期间（而非运动期间）酮血症程度的差异是由于第3组大鼠的肝丙二酰辅酶A较低。
