通过酶联免疫吸附测定法对鱼类传染性胰腺坏死病毒进行体外和体内检测。

In vitro and in vivo detection of infectious pancreatic necrosis virus in fish by enzyme-linked immunosorbent assay.

作者信息

Hattori M, Kodama H, Ishiguro S, Honda A, Mikami T, Izawa H

出版信息

Am J Vet Res. 1984 Sep;45(9):1876-9.

Abstract

A double antibody enzyme-linked immunosorbent assay (ELISA) was used to detect infectious pancreatic necrosis virus (IPNV). The ELISA detected VR299 strain of IPNV at a dose of 10 to 20 ng of purified IPNV protein or 10(4) TCID50 in tissue culture fluid. Specificity of ELISA was demonstrated by an ELISA inhibition test. The ELISA did not detect infectious hematopoietic necrosis virus. Normal cell culture fluid and virus-non-inoculated rainbow trout (Salmo gairdneri Richardson) homogenate did not react in the test system. The IPNV was detected in rainbow trout fry inoculated with IPNV. Although infective virus titer in fish decreased rapidly 1 week after inoculation, IPNV antigen was detected by ELISA for 15 days. The IPNV antigen was detected in the fish tissue after inactivation of infective virus. The ELISA is a rapid and reliable method for the diagnosis of IPNV infection.

摘要

采用双抗体酶联免疫吸附测定法（ELISA）检测传染性胰腺坏死病毒（IPNV）。该ELISA法能检测到剂量为10至20纳克纯化IPNV蛋白或组织培养液中10(4) 半数组织培养感染剂量（TCID50）的IPNV VR299株。通过ELISA抑制试验证明了ELISA的特异性。该ELISA法未检测到传染性造血器官坏死病毒。正常细胞培养液和未接种病毒的虹鳟（Salmo gairdneri Richardson）匀浆在检测系统中无反应。在接种IPNV的虹鳟鱼苗中检测到了IPNV。尽管接种1周后鱼体内的感染性病毒滴度迅速下降，但ELISA法在15天内都检测到了IPNV抗原。在感染性病毒灭活后的鱼组织中检测到了IPNV抗原。ELISA法是诊断IPNV感染的一种快速且可靠的方法。