Jansson C, Hansson O, Akerlund H E, Andreasson L E
Biochem Biophys Res Commun. 1984 Oct 15;124(1):269-76. doi: 10.1016/0006-291x(84)90947-1.
EPR measurements on inside-out thylakoids revealed that salt-washing, known to inhibit oxygen evolution and release a 23 and a 16 kDa protein, induced a Signal IIf and decreased the EPR signal from state S2. Readdition of the released 23 kDa protein restored the oxygen evolution and decreased the Signal IIf, but did not relieve the decrease in the state S2 signal. It is suggested that salt-washing inhibits the electron transfer from the oxygen-evolving site to Z, the physiological donor to P680. In inhibited photosystem II units lacking Signal IIf, Z+ is rapidly reduced, possibly by a modified S-cycle unable to evolve oxygen.
对内翻类囊体进行的电子顺磁共振（EPR）测量表明，已知盐洗会抑制氧气释放并释放出一种23 kDa和一种16 kDa的蛋白质，盐洗会诱导产生信号IIf并降低来自S2状态的EPR信号。重新添加释放出的23 kDa蛋白质可恢复氧气释放并降低信号IIf，但不能缓解S2状态信号的降低。有人提出，盐洗会抑制从放氧位点到Z（P680的生理供体）的电子转移。在缺乏信号IIf的受抑制光系统II单元中，Z+可能通过无法释放氧气的修饰S循环而迅速被还原。
