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单价和二价盐对菠菜光系统II制剂的氧气释放活性和低温多线电子顺磁共振光谱的影响。

The effects of mono- and divalent salts on the O2-evolution activity and low temperature multiline EPR spectrum of Photosystem II preparations from spinach.

作者信息

Blough N V, Sauer K

出版信息

Biochim Biophys Acta. 1984 Nov 26;767(2):377-81. doi: 10.1016/0005-2728(84)90208-1.

DOI:10.1016/0005-2728(84)90208-1
PMID:6093874
Abstract

The ability of salts to inhibit the O2-evolution activity of PS II preparations is shown to parallel closely the Hofmeister series, suggesting that inhibition is related to the solubility of the 16, 24 and 33 kDa proteins in these salt solutions. An examination of the effect of salt inactivation on the low temperature multiline EPR signal indicates that the release of either the 16 and 24 kDa proteins, or additionally the 33 kDa protein blocks or greatly reduces the efficiency of the advancement of the water-splitting complex to the S2-state; under some conditions, this inhibition is reversible.

摘要

盐抑制光系统II制剂放氧活性的能力与霍夫迈斯特序列密切平行，这表明抑制作用与这些盐溶液中16 kDa、24 kDa和33 kDa蛋白质的溶解度有关。对盐失活对低温多线EPR信号的影响进行的研究表明，16 kDa和24 kDa蛋白质的释放，或者另外33 kDa蛋白质的释放会阻止或大大降低水裂解复合物向S2态推进的效率；在某些条件下，这种抑制是可逆的。

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