Baranao J L, Hammond J M
Biochem Biophys Res Commun. 1984 Oct 30;124(2):484-90. doi: 10.1016/0006-291x(84)91579-1.
The effects of insulin-like growth factors (IGF-I and II) were compared with those of insulin in porcine granulosa cells in vitro with regard to stimulation of thymidine incorporation, facilitation of FSH-induced progesterone secretion, and displacement of [125I] IGF-I from specific receptors. In all three assays, the order of potency was IGF-I from specific receptors. In all three assays, the order of potency was IGF-I greater than IGF-II greater than insulin. However, the difference in potency was marginally significant in the mitogenic assay. These data are consistent with the presence of specific IGF-I receptors on these cells and involvement of these binding sites in regulation of granulosa cell replication and differentiation.
在体外培养的猪颗粒细胞中，就刺激胸苷掺入、促进促卵泡激素诱导的孕酮分泌以及从特异性受体上置换[125I]胰岛素样生长因子-I（IGF-I）而言，比较了胰岛素样生长因子（IGF-I和IGF-II）与胰岛素的作用。在所有这三种测定中，效力顺序为IGF-I大于IGF-II大于胰岛素。然而，在促有丝分裂测定中，效力差异仅具有边际显著性。这些数据与这些细胞上存在特异性IGF-I受体以及这些结合位点参与颗粒细胞复制和分化的调节相一致。
