Stastny J, Prasad R, Fosslien E
Clin Chem. 1984 Dec;30(12 Pt 1):1914-8.
Proteins from 25 specimens of malignant breast tissue were separated by "high-resolution" two-dimensional gel electrophoresis. The number of polypeptide spots detectable in individual gels varied from 259 to 471 (mean 347; SD 68) per milligram of wet tissue. Many polypeptide spots were not consistently present in all malignant tissues. Ten different in vivo polypeptides, observed neither in five non-malignant breast tissues nor in human milk, were present in more than 22 of the 25 cases of breast tumor tissues examined. Five of these polypeptides appeared to be identical to five in vitro polypeptides found in separations from cultured human ductal carcinoma cells. Further studies are required to verify the specificity of these tumor-associated proteins before they can be considered for breast tumor-specific in vitro diagnostic testing or targeting in selective in vivo anti-cancer therapy.
采用“高分辨率”二维凝胶电泳法对25份恶性乳腺组织样本中的蛋白质进行了分离。每毫克湿组织在各凝胶中可检测到的多肽斑点数量在259至471个之间（平均347个；标准差68个）。许多多肽斑点并非在所有恶性组织中都始终存在。在25例接受检查的乳腺肿瘤组织中，有22例以上存在10种不同的体内多肽，这些多肽在5份非恶性乳腺组织和人乳中均未观察到。其中5种多肽似乎与从培养的人导管癌细胞分离物中发现的5种体外多肽相同。在将这些肿瘤相关蛋白用于乳腺肿瘤特异性体外诊断检测或选择性体内抗癌治疗的靶向治疗之前，还需要进一步研究以验证其特异性。
