Lum L G, Seigneuret M C, Orcutt-Thordarson N, Froelich T L, Storb R
Diagn Immunol. 1983;1(3):179-87.
T and B cells from one short-term and nine long-term patients who had received HLA-identical bone marrow transplants for aplastic anemia or hematologic malignancy were studied for their ability to synthesize immunoglobulin after in vitro stimulation with pokeweed mitogen, Herpes simplex type 1 antigen, tetanus toxoid, or Epstein-Barr virus. Purified T, OKT4, OKT8, or B cells from patients were cocultured with normal T and/or B cells in the presence of the various stimulants. Multiple regulatory defects were observed in long-term patients with and without chronic graft-versus-host disease. The lymphocytes from the long-term healthy chimeras exhibited fewer defects in their ability to regulate in vitro immunoglobulin synthesis than those of patients with chronic graft-versus-host disease. These findings suggest that the presence of chronic graft-versus-host disease delays or impairs the "rematuration" of the immune system after bone marrow grafting and that the rematurational process occurs at the clonal level.
对一名接受过 HLA 相同骨髓移植治疗再生障碍性贫血或血液系统恶性肿瘤的短期患者以及九名长期患者的 T 细胞和 B 细胞进行了研究，以观察它们在用商陆有丝分裂原、单纯疱疹病毒 1 型抗原、破伤风类毒素或爱泼斯坦 - 巴尔病毒进行体外刺激后合成免疫球蛋白的能力。将患者的纯化 T 细胞、OKT4、OKT8 或 B 细胞与正常 T 细胞和/或 B 细胞在各种刺激物存在的情况下进行共培养。在有或没有慢性移植物抗宿主病的长期患者中观察到多种调节缺陷。长期健康嵌合体的淋巴细胞在调节体外免疫球蛋白合成能力方面表现出的缺陷比患有慢性移植物抗宿主病的患者少。这些发现表明，慢性移植物抗宿主病的存在会延迟或损害骨髓移植后免疫系统的“再成熟”，并且再成熟过程发生在克隆水平。
