Tsang C P, Grunder A A
Endocrinology. 1984 Dec;115(6):2170-5. doi: 10.1210/endo-115-6-2170.
Evidence is presented that in vitamin D deficiency there are aberrations of estrogen metabolism in the laying hen. Five White Leghorn hens, 35 weeks of age, all laying soft shelled eggs as a result of complete withdrawal of supplemental vitamin D3 (D3) from the diet, were found to have a 3-fold higher molar ratio of plasma estradiol-17 beta-3-sulfate (E2 beta-3S) to estradiol-17 beta (E2 beta) compared to five control laying hens fed a D3-supplemented diet. A time course tracer study with [3H]E2 beta administered iv revealed an abnormally high accumulation of [3H]E2 beta-3S in plasma, and concomitantly, a markedly reduced formation of [3H]estradiol-17 alpha-3-sulfate [( 3H]E2 alpha-3S) compared with the control hens. This observation suggests a defect in the conversion of [3H]E2 beta-3S to [3H]E1S, previously shown to be a major pathway for E2 beta metabolism (E2 beta E2 beta-3S in equilibrium E1S E2 alpha-3S). As a result, the MCR of [3H]E2 beta from plasma of the D3-deficient hens was about 15% below normal. The calculated production rate of E2 beta was reduced even more, by 60%. However, the reduced production rate could have been related to the lengthening of the ovulatory cycle due to vitamin D deficiency. It is concluded that vitamin D deficiency affects the biosynthesis and catabolism of E2 beta in the laying hen.
有证据表明，产蛋母鸡维生素D缺乏时雌激素代谢会出现异常。5只35周龄的白来航母鸡，由于日粮中完全不添加维生素D3（D3）而全部产软壳蛋，与5只饲喂添加D3日粮的对照产蛋母鸡相比，发现其血浆雌二醇-17β-3-硫酸盐（E2β-3S）与雌二醇-17β（E2β）的摩尔比高3倍。静脉注射[3H]E2β进行的时间进程示踪研究显示，血浆中[3H]E2β-3S异常高积累，同时，与对照母鸡相比，[3H]雌二醇-17α-3-硫酸盐[（3H]E2α-3S）的形成明显减少。这一观察结果表明，[3H]E2β-3S向[3H]E1S的转化存在缺陷，此前已证明这是E2β代谢的主要途径（E2β⇌E2β-3S⇌E1S⇌E2α-3S）。因此，维生素D缺乏母鸡血浆中[3H]E2β的代谢清除率比正常低约15%。计算得出的E2β生成率下降得更多，达60%。然而，生成率降低可能与维生素D缺乏导致排卵周期延长有关。结论是，维生素D缺乏会影响产蛋母鸡中E2β的生物合成和分解代谢。
