Galdiero F, Tufano M A, Sommese L, Folgore A, Tedesco F
Infect Immun. 1984 Nov;46(2):559-63. doi: 10.1128/iai.46.2.559-563.1984.
The effect of porins purified from Salmonella typhimurium on the complement system was investigated both in vitro and in vivo. Incubation of porins with either human or guinea pig serum resulted in the consumption of the total complement activity when an amount of porins ranging from 8 to 10 micrograms per 100 microliters of serum was used. The activation of the complement system was temperature dependent, suggesting an active process rather than passive adsorption of the complement components by porins. In addition, the activation had a fast kinetic and proceeded mainly through the classical pathway. This conclusion is supported by the consumption of C1s and C4 in normal human serum treated with porins and also by the depletion of C3 activity in the C1s-deficient serum which was marked only when purified C1s was added to the serum before incubation with porins. Injection of 100 micrograms of porins into guinea pigs induced profound complement consumption at 6 h postinjection that persisted up to 12 h. We conclude from this study that porins can effectively contribute to complement activation and to subsequent biological events induced by gram-negative bacteria.
研究了从鼠伤寒沙门氏菌中纯化的孔蛋白在体外和体内对补体系统的影响。当每100微升血清中使用8至10微克的孔蛋白量时，将孔蛋白与人或豚鼠血清一起孵育会导致总补体活性的消耗。补体系统的激活是温度依赖性的，这表明是一个主动过程，而不是孔蛋白对补体成分的被动吸附。此外，激活具有快速动力学，主要通过经典途径进行。用孔蛋白处理的正常人血清中C1s和C4的消耗以及C1s缺陷血清中C3活性的消耗支持了这一结论，只有在与孔蛋白孵育前向血清中加入纯化的C1s时，C1s缺陷血清中C3活性的消耗才明显。向豚鼠注射100微克孔蛋白在注射后6小时引起深刻的补体消耗，这种消耗持续到12小时。我们从这项研究中得出结论，孔蛋白可以有效地促进补体激活以及革兰氏阴性菌诱导的后续生物学事件。
