Cossu M, Lantini M S, Puxeddu P, Riva A
Histochemistry. 1984;81(3):221-5. doi: 10.1007/BF00495631.
K+ -dependent p-nitrophenylphosphatase (pNPPase) and Ca++ -stimulated adenosine triphosphatase (ATPase) activities were studied in human parotid and submandibular glands using cytochemical methods at the ultrastructural level. In both glands, only the striated-duct epithelium showed K+ -pNPPase reaction product, thereby indicating the localization of Na+, K+ -ATPase. The precipitate was concentrated on the deep invaginations of the basolateral plasma membranes, in close association with their cytoplasmic surface. Ca++ -ATPase activity was also found on the basolateral plasma membranes, but two striking differences from the K+ -pNPPase distribution were observed: firstly, Ca++ -ATPase appeared in both acinar and ductal cells, and secondly, it was localized on the outer side of the plasma membranes.
利用超微结构水平的细胞化学方法，对人腮腺和颌下腺中钾离子依赖性对硝基苯磷酸酶（pNPPase）和钙离子刺激的三磷酸腺苷酶（ATPase）活性进行了研究。在这两种腺体中，只有纹状管上皮显示出钾离子-pNPPase反应产物，从而表明钠钾ATP酶的定位。沉淀物集中在基底外侧质膜的深陷处，与其细胞质表面紧密相连。在基底外侧质膜上也发现了钙离子ATP酶活性，但观察到与钾离子-pNPPase分布有两个显著差异：首先，钙离子ATP酶出现在腺泡细胞和导管细胞中；其次，它定位在质膜的外侧。
