• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

大肠杆菌K-12 metA基因的调控区域。

Regulatory region of the metA gene of Escherichia coli K-12.

作者信息

Michaeli S, Mevarech M, Ron E Z

出版信息

J Bacteriol. 1984 Dec;160(3):1158-62. doi: 10.1128/jb.160.3.1158-1162.1984.

DOI:10.1128/jb.160.3.1158-1162.1984
PMID:6094503
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC215834/
Abstract

Transcription of the metA gene of Escherichia coli K-12 is from a promoter which is under methionine control and is located next to a region which has an extensive sequence homology with the operator regions of the metBL and metF genes. However, in the metA gene there is a second transcription start point which is located 74 base pairs upstream and which is independent of the intracellular methionine concentration.

摘要

大肠杆菌K-12的metA基因转录自一个受甲硫氨酸调控的启动子，该启动子位于一个与metBL和metF基因的操纵区具有广泛序列同源性的区域附近。然而，在metA基因中还有第二个转录起始点，它位于上游74个碱基对处，且与细胞内甲硫氨酸浓度无关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be57/215834/334ebcc48edd/jbacter00229-0337-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be57/215834/334ebcc48edd/jbacter00229-0337-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be57/215834/334ebcc48edd/jbacter00229-0337-a.jpg

相似文献

1
Regulatory region of the metA gene of Escherichia coli K-12.大肠杆菌K-12 metA基因的调控区域。
J Bacteriol. 1984 Dec;160(3):1158-62. doi: 10.1128/jb.160.3.1158-1162.1984.
2
Regulation of in vivo transcription of the Escherichia coli K-12 metJBLF gene cluster.大肠杆菌K-12 metJBLF基因簇的体内转录调控
J Bacteriol. 1986 Mar;165(3):671-7. doi: 10.1128/jb.165.3.671-677.1986.
3
An Escherichia coli gene divergently transcribed from a promoter overlapping the metA promoter.
FEMS Microbiol Lett. 1997 Sep 15;154(2):219-22. doi: 10.1111/j.1574-6968.1997.tb12647.x.
4
Homoserine O-acetyltransferase, involved in the Leptospira meyeri methionine biosynthetic pathway, is not feedback inhibited.参与迈耶氏钩端螺旋体蛋氨酸生物合成途径的高丝氨酸O-乙酰转移酶不受反馈抑制。
J Bacteriol. 1997 Jul;179(13):4396-8. doi: 10.1128/jb.179.13.4396-4398.1997.
5
Nucleotide sequence of the metA gene encoding homoserine trans-succinylase in Escherichia coli.编码大肠杆菌中高丝氨酸转琥珀酰酶的metA基因的核苷酸序列。
Nucleic Acids Res. 1989 Apr 11;17(7):2856. doi: 10.1093/nar/17.7.2856.
6
Heat shock-dependent transcriptional activation of the metA gene of Escherichia coli.大肠杆菌metA基因的热休克依赖性转录激活。
J Bacteriol. 1995 Mar;177(5):1374-9. doi: 10.1128/jb.177.5.1374-1379.1995.
7
Enzyme-catalyzed acylation of homoserine: mechanistic characterization of the Escherichia coli metA-encoded homoserine transsuccinylase.酶催化的高丝氨酸酰化作用：大肠杆菌metA编码的高丝氨酸转琥珀酰酶的机制表征
Biochemistry. 1999 Oct 26;38(43):14416-23. doi: 10.1021/bi991710o.
8
Regulation of homoserine O-succinyltransferase for efficient production of L-methionine in engineered Escherichia coli.工程化大肠杆菌中高丝氨酸O-琥珀酰转移酶的调控以高效生产L-甲硫氨酸
J Biotechnol. 2020 Feb 10;309:53-58. doi: 10.1016/j.jbiotec.2019.12.018. Epub 2019 Dec 28.
9
Identification of a functional promoter for the Escherichia coli gdhA gene and its regulation.大肠杆菌gdhA基因功能性启动子的鉴定及其调控
Gene. 1988 Nov 30;71(2):233-46. doi: 10.1016/0378-1119(88)90040-6.
10
Adaptation of Escherichia coli to elevated temperatures: the metA gene product is a heat shock protein.大肠杆菌对高温的适应性：metA基因产物是一种热休克蛋白。
Antonie Van Leeuwenhoek. 1990 Oct;58(3):169-74. doi: 10.1007/BF00548929.

引用本文的文献

1
Methionine Limitation Impairs Pathogen Expansion and Biofilm Formation Capacity.蛋氨酸限制会损害病原体的扩张和生物膜形成能力。
Appl Environ Microbiol. 2019 Apr 18;85(9). doi: 10.1128/AEM.00177-19. Print 2019 May 1.
2
Stabilization of homoserine-O-succinyltransferase (MetA) decreases the frequency of persisters in Escherichia coli under stressful conditions.高丝氨酸-O-琥珀酰基转移酶（MetA）的稳定化降低了应激条件下大肠杆菌中持留菌的频率。
PLoS One. 2014 Oct 17;9(10):e110504. doi: 10.1371/journal.pone.0110504. eCollection 2014.
3
Potential for development of an Escherichia coli-based biosensor for assessing bioavailable methionine: a review.

本文引用的文献

1
THE NH2-TERMINAL RESIDUES OF THE PROTEINS FROM CELL-FREE EXTRACTS OF E. COLI.大肠杆菌无细胞提取物中蛋白质的氨基末端残基
J Mol Biol. 1963 Nov;7:483-96. doi: 10.1016/s0022-2836(63)80096-0.
2
Construction and physical mapping of plasmids containing the MetA gene of Escherichia coli K-12.含有大肠杆菌K-12 MetA基因的质粒的构建及物理图谱分析
Mol Gen Genet. 1981;182(2):349-54. doi: 10.1007/BF00269682.
3
Structure of the metJBLF cluster in Escherichia coli K12. Sequence of the metB structural gene and of the 5'- and 3'-flanking regions of the metBL operon.
基于大肠杆菌的生物传感器评估生物可利用蛋氨酸的发展潜力：综述。
Sensors (Basel). 2010;10(4):3562-84. doi: 10.3390/s100403562. Epub 2010 Apr 8.
4
Linkage map of Escherichia coli K-12, edition 10: the traditional map.大肠杆菌K-12连锁图谱，第10版：传统图谱。
Microbiol Mol Biol Rev. 1998 Sep;62(3):814-984. doi: 10.1128/MMBR.62.3.814-984.1998.
5
Molecular characterization and sequence of a methionine biosynthetic locus from Pseudomonas syringae.丁香假单胞菌蛋氨酸生物合成基因座的分子特征与序列分析
J Bacteriol. 1998 Sep;180(17):4497-507. doi: 10.1128/JB.180.17.4497-4507.1998.
6
Homoserine O-acetyltransferase, involved in the Leptospira meyeri methionine biosynthetic pathway, is not feedback inhibited.参与迈耶氏钩端螺旋体蛋氨酸生物合成途径的高丝氨酸O-乙酰转移酶不受反馈抑制。
J Bacteriol. 1997 Jul;179(13):4396-8. doi: 10.1128/jb.179.13.4396-4398.1997.
7
Compilation of E. coli mRNA promoter sequences.大肠杆菌信使核糖核酸启动子序列的汇编。
Nucleic Acids Res. 1993 Apr 11;21(7):1507-16. doi: 10.1093/nar/21.7.1507.
8
Heat shock-dependent transcriptional activation of the metA gene of Escherichia coli.大肠杆菌metA基因的热休克依赖性转录激活。
J Bacteriol. 1995 Mar;177(5):1374-9. doi: 10.1128/jb.177.5.1374-1379.1995.
9
Analysis of E. coli promoter sequences.大肠杆菌启动子序列分析。
Nucleic Acids Res. 1987 Mar 11;15(5):2343-61. doi: 10.1093/nar/15.5.2343.
10
Operator-constitutive mutations of the Escherichia coli metF gene.大肠杆菌metF基因的操纵子组成型突变
J Bacteriol. 1987 Feb;169(2):670-4. doi: 10.1128/jb.169.2.670-674.1987.
大肠杆菌K12中metJBLF基因簇的结构。metB结构基因以及metBL操纵子5'和3'侧翼区域的序列。
J Biol Chem. 1983 Dec 25;258(24):14868-71.
4
Nucleotide sequence of metF, the E. coli structural gene for 5-10 methylene tetrahydrofolate reductase and of its control region.大肠杆菌5-10亚甲基四氢叶酸还原酶结构基因metF及其调控区的核苷酸序列。
Nucleic Acids Res. 1983 Oct 11;11(19):6723-32. doi: 10.1093/nar/11.19.6723.
5
Molecular basis for modulated regulation of gene expression in the arginine regulon of Escherichia coli K-12.大肠杆菌K-12精氨酸调节子中基因表达调控的分子基础。
Nucleic Acids Res. 1983 Aug 11;11(15):5007-19. doi: 10.1093/nar/11.15.5007.
6
Linkage map of Escherichia coli K-12, edition 7.大肠杆菌K-12连锁图谱，第7版。
Microbiol Rev. 1983 Jun;47(2):180-230. doi: 10.1128/mr.47.2.180-230.1983.
7
Evidence for two functional gal promoters in intact Escherichia coli cells.完整大肠杆菌细胞中两个功能性半乳糖启动子的证据。
J Biol Chem. 1981 Nov 25;256(22):11905-10.
8
Sequencing end-labeled DNA with base-specific chemical cleavages.通过碱基特异性化学切割对末端标记的DNA进行测序。
Methods Enzymol. 1980;65(1):499-560. doi: 10.1016/s0076-6879(80)65059-9.
9
Differential stringent control of the tandem E. coli ribosomal RNA promoters from the rrnA operon expressed in vivo in multicopy plasmids.多拷贝质粒在体内表达时，对rrnA操纵子中串联的大肠杆菌核糖体RNA启动子的差异严格控制。
Cell. 1983 Apr;32(4):1337-46. doi: 10.1016/0092-8674(83)90314-8.
10
In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals.将具有酶活性的β-半乳糖苷酶片段与外源蛋白质的氨基末端片段连接的体外基因融合：用于检测和克隆翻译起始信号的大肠杆菌质粒载体。
J Bacteriol. 1980 Aug;143(2):971-80. doi: 10.1128/jb.143.2.971-980.1980.